Histochemical demonstration of non-specific esterases and non-specific acid phosphatases using menadiol substrates

Acta histochemica
A Dikow, R Gossrau

Abstract

Although many synthetic substrates and methods are available for the histochemical detection of non-specific esterases and non-specific acid phosphatase, there are still further possibilities to investigate these hydrolases histochemically. This was shown for menadiol diacetate and menadiol diphosphate using tetrazolium salt, simultaneous azo-dye as well as metal salt methods in many rat tissues. In comparison, the azo-dye procedure with various Fast salts or hexazonium Pararosaniline or New Fuchsin delivered less satisfactory results; precisely localized stain in sufficient amounts was obtained for non-specific esterases using nitro BT, tetranitro BT or benzothiazolystyrylphthalhydrazidyl tetrazolium (BSPT) and for non-specific acid phosphatase with BSPT in the tetrazolium salt method or using cerium ions for phosphate trapping in the diaminobenzidine-nickel-hydrogen peroxide procedure.

References

May 1, 1979·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·T A PlompR A Maes
Oct 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G N JongesR Gossrau
Jul 1, 1985·The Histochemical Journal·R Gossrau
Jan 1, 1984·Critical Reviews in Clinical Laboratory Sciences·R G Hamilton, N F Adkinson

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Citations

Jan 24, 2004·Journal of Experimental Botany·Sébastien BaudChristine Rochat

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