PMID: 7537145Jan 1, 1995Paper

Histochemical detection of carbohydrates of Blastocystis hominis

The Journal of Eukaryotic Microbiology
H YoshikawaY Enose

Abstract

The carbohydrates of Blastocystis hominis were detected by histochemical techniques using light and electron microscopy. B. hominis, fixed with various fixatives, followed by treatment with detergents, were stained with periodic acid-Schiff (PAS) or alcian blue (AB). Intense PAS reactions were observed in cells fixed with glutaraldehyde or 1/2 Karnovsky fixative. The cells fixed with other fixatives showed weak or no reactions with PAS staining. Similar results were seen in the case of AB stain. These results indicated that, depending on the fixative used, B. hominis contained PAS- or AB-reactive carbohydrates. At the electron microscopic level, ultrathin sections of B. hominis were stained with periodic acid methenamine silver (PA-MS) or periodic acid thiocarbohydrazide-silver proteinate (PA-TCH-SP) staining techniques. Intense, positive reactions with PA-MS or PA-TCH-SP were observed on the central vacuole, Golgi apparatus, and cytoplasmic vesicles. The filamentous layer showed moderate reactions with PA-MS, whereas in PA-TCH-SP stain, it was stained more densely. The staining intensity of the central vacuole varied from cell to cell. The presence of membrane fusions of the cytoplasmic vesicles with the central vacuole indica...Continue Reading

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Citations

Oct 14, 2004·Parasitology Research·Hisao YoshikawaIsao Kimata
Mar 18, 2005·Parasitology Research·Aya TanizakiIsao Kimata
May 1, 1996·International Journal for Parasitology·M D LanuzaR Borrás
Apr 1, 1996·International Journal for Parasitology·S W TanM Singh
Jun 14, 2002·International Journal for Parasitology·Kevin S W TanEu Hian Yap
Aug 1, 1997·International Journal for Parasitology·S W TanE H Yap
Aug 21, 2013·Tropical Parasitology·Subhash Chandra Parija, Ss Jeremiah
Jun 14, 2002·Veterinary Parasitology·Niichiro AbeHisao Yoshikawa
Jan 26, 2007·Parasitology International·Aya IguchiIsao Kimata
Jun 5, 2004·Veterinary Parasitology·Hisao YoshikawaNaoko Miyamoto
May 23, 2015·Parasitology Research·Robyn NagelRebecca J Traub
Aug 22, 2018·Genes·John Anthony Yason, Kevin Shyong Wei Tan
Mar 23, 2002·The Journal of Eukaryotic Microbiology·Nobuko ArisueMasami Hasegawa
Apr 4, 2003·The Journal of Eukaryotic Microbiology·Hisao YoshikawaMulkit Singh
Apr 4, 2000·Journal of Clinical Microbiology·H YoshikawaY Takahashi
Oct 1, 1996·Clinical Microbiology Reviews·D J Stenzel, P F Boreham

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