PMID: 9554581Apr 29, 1998Paper

Histochemical determination of stereoselectivity of esterases in normal pancreas and pancreatic tubular adenocarcinoma of hamsters

Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission
Y YamazakiS Oka

Abstract

Esterases in normal hamster pancreas and pancreatic tubular adenocarcinoma of ductal origin induced by N-nitrosobis(2-oxopropyl)amine were stained in cryostat sections with mixtures of a diazonium salt (fast blue RR) and with each of the enantiomers of alpha-naphthyl N-methoxycarbonylalaninate, N-methoxycarbonylvalinate, and N-acetylprolinate. Azo coupling of alpha-naphthol formed by enzymatic hydrolysis with the diazonium salt gives an azo dye that indicates the presence and amount of the enzyme activity in situ. Comparison between the color intensities obtained with each of the enantiomers of a chiral alpha-naphthyl ester shows the stereoselectivity, or enantiomeric preference, of the enzyme. Esterases in acinar cells of the normal pancreas showed slight stereoselectivity for N-methoxycarbonylalaninate, while esterases in fat cells scattered throughout the exocrine pancreas showed high stereoselectivity for (R)-N-acetylprolinate. These esterase activities were not found in the tumor, but another prominent esterase activity with high stereoselectivity for (S)-N-methoxycarbonylvalinate was found. Similar results were obtained by staining after polyacrylamide gel electrophoresis showing that the bands of esterases in the adenoca...Continue Reading

References

Jan 1, 1973·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C Y LiL T Yam
Nov 1, 1995·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·M MohiuddinR Cantor
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Dec 1, 1994·Archives of Biochemistry and Biophysics·E W MorganA Parkinson
Sep 1, 1995·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·A S AfifyH Okuno

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Citations

Feb 12, 2014·BMC Cancer·Christopher A McGoldrickWilliam L Stone
Feb 4, 2021·British Journal of Cancer·Romika KumariCaroline A Heckman

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