Histochemical localization of neutral proteases released during development of rat periradicular lesion

Archives of Oral Biology
Masahito TsujiHiroshi Nakamura

Abstract

The purpose of the present study was to examine the role of various neutral proteases released during the development of periradicular lesion. This lesion produced by pulpal exposure of mandibular first molar in rat. The histological and histometrical changes in periapical tissue examined. The presence of neutrophil elastase, cathepsin G, collagenase 2, gelatinase B, and secretory leukocyte protease inhibitor (SLPI) was immunohistochemically evaluated in the periapical tissue. After pulpal exposure, some inflammatory cells were present in the periapical tissue at 7 days, and periapical inflammation gradually increased. Alveolar bone resorption observed after 14 days and apical abscess found after 21 days. After 14 days, the area of periradicular lesion significantly increased compared from normal one (p<0.05). Neutrophil elastase, cathepsin G, collagenase 2, and gelatinase B were detected around the root apex at 14 days, then these proteases increased gradually and demonstrated in and around the abscess at 21 and 28 days. Morphologically, these protease-expressing cells are almost polymorphic and polynuclear shaped after 14 days. These cells significantly increased after 14 days compared from normal one (p<0.05). However, SLPI ...Continue Reading

References

Nov 1, 1993·Journal of Dental Research·C J CootaucoR K Nauman
Oct 1, 1994·Oral Surgery, Oral Medicine, and Oral Pathology·P StashenkoS M Yu
Jun 1, 1994·Oral Surgery, Oral Medicine, and Oral Pathology·M YamasakiH Nakamura
Jan 1, 1994·Journal of Endodontics·M YamasakiY Kameyama
Feb 19, 1998·Biochimica Et Biophysica Acta·A D PembertonH R Miller
Aug 19, 2000·Cellular and Molecular Life Sciences : CMLS·N JohanssonV M Kähäri
Nov 30, 2000·Developmental Immunology·H SchorO Lider
Jan 12, 2001·Oral Microbiology and Immunology·I J Márton, C Kiss
Jan 18, 2003·Contributions to Microbiology·Hans Flodgaard
Sep 25, 2003·Connective Tissue Research·Michel GoldbergSuzanne Menashi
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Karsten GronertCharles N Serhan
Nov 10, 2004·Oral Diseases·T SorsaT Salo
Mar 18, 2005·Annual Review of Immunology·Anthony W Segal
Mar 26, 2005·The Biochemical Journal·Lyle J FreemanCay M Kielty
Mar 29, 2005·Angiology·Nikolaos P KadoglouChristos D Liapis
Apr 20, 2005·Advances in Protein Chemistry·Cay M KieltyClair Baldock
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen
Oct 27, 2005·Journal of Endodontics·Nilgun Ozlem AlptekinTamer Ataoglu
Jan 18, 2006·Journal of Endodontics·Masahiro YamasakiHiroshi Nakamura
Apr 25, 2006·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·Akihiro IwamaHiroshi Nakamura
Jun 2, 2007·Internal Medicine·Tadatomo KodamaSeiichi Kitagawa
Jun 15, 2007·Journal of Periodontal Research·Y UjiieM Fukae
Aug 23, 2008·Journal of Endodontics·Taisuke MorimotoHiroshi Nakamura
Nov 11, 2008·Archives of Oral Biology·J L BainR B Johnson
Jun 6, 2009·Archives of Oral Biology·Mauro V CorottiJosé M Granjeiro

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Citations

Apr 29, 2014·Journal of Endodontics·Chunyan WanZhi Chen
Jan 28, 2014·Journal of Endodontics·Ildikó J Márton, Csongor Kiss
Jul 6, 2017·Brazilian Oral Research·Pâmella Recco ÁlvaresAna Paula Veras Sobral
Oct 27, 2018·Brazilian Oral Research·Brenda Paula Figueiredo de Almeida Gomes, Daniel Rodrigo Herrera

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