PMID: 58938Mar 1, 1976

Histochemical staining in lymphocyte suspensions complexed with beta-galactosidase as an antigen. Unspecific staining of spleen cells by indigo

Journal of Immunological Methods
L Dankwarth, W Messer

Abstract

Spleen cells from mice immunized with E. coli beta-galactosidase were incubated with the enzyme and with 5-bromo-4-chloroindolyl-beta-galactosidase as a histochemical substrate. Some cells are stainable in this reaction, however, not all of them carry specific receptors for beta-galactosidases. Some spleen cells can take up dye and thereby become unspecifically stained. This system is therefore not suitable for the detection of antigen binding to cells.

References

Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, D R Cox
Feb 20, 1970·Biochemical and Biophysical Research Communications·D GivolM Wilchek

Related Concepts

Metazoa
Binding Sites, Antibody
Galactosidase
Lymphoid Cells
Spleen
Staining and Labeling
Mouse, Swiss

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