PMID: 107709Jan 1, 1978

Histochemical technique to detect choline-containing lipids

Acta histochemica
W A Hadler, S R Silveira

Abstract

An almost specific and highly sensitive technique to detect histochemically choline-containing lipids is proposed. This technique is based on the effect of Hg++ and phosphomolybdic ion on the choline, that yields insoluble complexes able to react with diphenylcarbazide producing a deep blue or violet stain. The specificity and the sensitivity of this technique was investigated on filter paper strips loaded with choline-containing lipids, choline-free lipids, steroids, vitamins, proteins or choline hydrochloride. The results show that the choline-containing substances react positively. The choline-free substances react negatively except calciferol and vitamin A that show weakly positive results. This technique used on tissue sections appears very suitable to histochemical purposes.

References

Oct 1, 1955·The Journal of Pathology and Bacteriology·A G E PEARSE
Apr 1, 1959·The Journal of Pathology and Bacteriology·C W ADAMS
Jan 1, 1964·Journal of Atherosclerosis Research·C J BOETTCHER, E BOELSMA-VANHOUTE
Jun 15, 1950·Experientia·E CHARGAFFJ KREAM

Citations

Jul 1, 1997·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·C S PaivaA T Yamada

Related Concepts

Adrenal Glands
Brain Chemistry
Felis catus
Choline Hydroxide
Canis familiaris
Cytochemistry
Lipids
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