PMID: 3744895Jan 1, 1986Paper

Histochemistry of glycosaminoglycans in cartilage ground substance. Alcian-blue staining and lectin-binding affinities in semithin Epon sections

Histochemistry
R MallingerP Böck

Abstract

The critical-electrolyte-concentration staining method using Alcian blue (AB) was applied to etched semithin Epon-embedded sections. The distribution of various glycosaminoglycans (GAGs) was studied in hyaline, elastic, cellular and fibrous cartilage obtained from humans and rodents. The staining patterns in semithin sections were found to correspond to those obtained using paraffin-embedded material. Lectin histochemistry was performed on consecutive sections. The following peroxidase-labelled lectins were used: Ricinus communis A I, Arachis hypogaea, Ulex europaeus A I, Triticum vulgaris, Helix pomatia, Limax flavus, and concanavalin A. The lectin-binding capacity of cartilaginous ground substance was found to be low, as was expected on account of the few free sugar residues of GAGs. Chondroitin sulphate, the most widely distributed GAG, did not exhibit lectin staining. The lectin-binding sites (positive staining for all lectins tested except H. pomatia) observed corresponded to areas positive for keratan sulphate, as shown by AB staining in preceding or following sections. The pronounced lectin binding seen in cellular structures and the inner territorial matrix regions is considered to be due to higher concentrations of oli...Continue Reading

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Citations

Jan 1, 1991·Anatomy and Embryology·J Milaire
Jan 1, 1988·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M SchünkeW Müller-Ruchholtz
May 19, 2007·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·M B Walker, C B Kimmel
Jan 1, 1987·Histochemistry·K O Söderström
Jan 1, 1994·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·U Schumacher, E Adam
Nov 19, 2014·Journal of Morphology·Horst Erich KönigPeter Böck
Jan 1, 1988·The American Journal of Anatomy·R Mallinger, L Stockinger
Apr 19, 2013·Reproduction in Domestic Animals = Zuchthygiene·C G BarbeitoN R Salvetti
Nov 24, 1999·Osteoarthritis and Cartilage·F P Paulsen, B N Tillmann

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