Jan 1, 1976

Histochemistry of normal and diseased human lymph nodes

Acta Anatomica
M T RakhawyA M Zakaria

Abstract

Gomori's metal precipitate technique was used to demonstrate the phosphatase activity of the human cervical lymph node in health and disease, using four different phosphate esters (sodium beta-glycerophosphate and adenosine triphosphate at pH 9, riboflavin 5'-phosphate at pH 9.2 and 5'-monophosphoric acid at pH 8.3). In fetal lymph nodes, using 5'-monophosphoric acid, an outstanding positive activity was noticed in the lymphatic follicles. With the other three substrates there was either no nodular reaction or just a narrow rim of positive activity around the follicles, the internodular tissue being negative with all four substrates used. With chronic non-specific lymphadenitis the enzyme hydrolysing the three substrates (beta-glycerophosphate, riboflavin 5'-phosphate and adenosine triphosphate) began to make their appearance. It seems that with lymphadenitis, a qualitative change of the phosphatase activity takes place. A special characteristic pattern of phosphatase activity has been described in both 'early' and 'caseating' tuberculous lymphadenitis. In malignant lymphomas it was noticed that no activity was encountered with any of the four substrates in reticulum cell sarcoma. However, in lymphosarcoma a positive activity w...Continue Reading

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Mentioned in this Paper

Lymphoma, Non-Hodgkin
Phosphoric Monoester Hydrolases
Flavin Mononucleotide
Phosphate Measurement
Riboflavin
Lymph Nodes
Histocytochemistry
Phosphoric Monoester Hydrolase Activity
Ovarian Follicle
Cervical Lymph Node Group

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