Histocompatibility requirements for T cell help in specific in vitro antibody responses to influenza virus by human blood lymphocytes

European Journal of Immunology
R Callard, C M Smith

Abstract

Specific antibody responses to influenza virus were obtained in vitro from human blood mononuclear cells (PBM). The response was T cell-dependent, as shown by separation of PBM into E rosette-positive (E+) and -negative (E-) populations. The histocompatibility requirements for T-B cells interactions in this response were analyzed by recombining E- and E+ fractions from donors with varying degrees of HLA compatibility. No antibody formation was obtained from any allogeneic combination except for the special case of HLA identical siblings. As these experiments included combinations with shared or identical HLA-DR specificities, it was unlikely that genetic restriction alone could account for the failure of T-B cell collaboration. Evidence that suppression was responsible for the lack of antibody formation was obtained from experiments in which allogeneic E+ cells profoundly depressed specific antibody responses of intact PBM. In contrast, no such suppression was seen in pokeweed mitogen-driven polyclonal Ig synthesis for which there are no major histocompatibility complex requirements for T cell help. The suppressor activity of allogeneic E+ cells was found to be radiation-sensitive. By irradiating E+ cells, it was, therefore, po...Continue Reading

References

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Citations

Jan 1, 1981·Journal of Immunological Methods·E D ZandersR E Callard
May 1, 1987·Annales De L'Institut Pasteur. Immunology·D JuyJ P Lévy
Nov 1, 1982·The Journal of Experimental Medicine·R L RaisonA Basten
Aug 1, 1983·The Journal of Clinical Investigation·H C LaneA S Fauci
Oct 7, 2004·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Robert LambkinColin Gelder
Sep 1, 1990·Scandinavian Journal of Immunology·K KristenssonR Carlsson
Sep 1, 1986·European Journal of Immunology·R E CallardR J Levinsky
Mar 1, 1982·European Journal of Immunology·R E CallardP C Beverley
Jan 1, 1985·European Journal of Immunology·R E CallardG W McCaughan
Jun 1, 1982·European Journal of Immunology·A LanzavecchiaF Celada

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