PMID: 2112601Jan 1, 1990Paper

Histoenzymological light and electron microscopic localization of carbonic anhydrase in the rat superficial pineal gland

Journal of Pineal Research
R V Krstić, D Nicolas

Abstract

A histoenzymological method for demonstration of carbonic anhydrase (CA) was used for cryostat sections of superficial rat pineal gland. Light and electron microscopic analysis showed a high concentration of this enzyme in stellate cells, particularly heavily distributed under the organ capsule and scattered in the parenchyma, where they form a widespread three-dimensional network. Most of the pericapillary spaces were lined by CA-reactive cells. The reaction product, in the form of strongly electron-dark precipitates, was localized in the cytoplasmic matrix, whereas organelles, except for some mitochondrial intracristal spaces, were CA-negative. On the basis of localization and morphology of CA-positive cells, we conclude that these represent glial (interstitial) cells.

References

Jan 1, 1987·The International Journal of Biochemistry·H F Deutsch
Sep 1, 1988·The Journal of Comparative Neurology·W Cammer, F A Tansey
Jan 1, 1984·Annals of the New York Academy of Sciences·G Lönnerholm
Feb 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·V WongL Guth
Apr 1, 1963·Journal of Neurochemistry·T NISHIMURAK NISHINUMA

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