Histologic, serologic, and molecular analysis of persistent ehrlichiosis in a murine model

The American Journal of Pathology
J P OlanoDavid H Walker

Abstract

Human monocytotropic ehrlichiosis caused by Ehrlichia chaffeensis was reported in 1987. An animal model to study acute fatal ehrlichiosis in mice that has been developed closely resembles the fatal form of human monocytotropic ehrlichiosis. However, animal models for persistent infection in the genus Ehrlichia in immunocompetent mice have not been characterized. We report the histopathological progression of Ehrlichia muris infection in immunocompetent mice (AKR and C57BL/6 strains) correlated with their antibody response determined by indirect immunofluorescence and Western immunoblotting, and the distribution and quantity of the ehrlichial load by immunohistochemistry, polymerase chain reaction (PCR), and real-time PCR in lungs, liver, and spleen. Mild to moderate correlation was observed between histopathological grading in these organs and relative ehrlichial loads. The highest ehrlichial loads were present between days 4 and 14 after infection. E. muris was detected in tissues examined up to 150 days after infection by real-time PCR. Analysis of the serological response revealed several immunodominant antigens, including 200-, 180-, 100-, 73/75-, 45-, and 28-kd proteins. In conclusion, we have provided for the first time a...Continue Reading

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Citations

Oct 28, 2011·Clinical and Vaccine Immunology : CVI·Patricia A Crocquet-ValdesDavid H Walker
Oct 7, 2009·Infection and Immunity·Nagaraja R ThirumalapuraNahed Ismail
Jul 29, 2009·Infection and Immunity·Bisweswar NandiGary M Winslow
Apr 18, 2007·Infection and Immunity·Koshiro Miura, Yasuko Rikihisa
Jul 30, 2010·PloS One·Young-Sang KohKoichi S Kobayashi
Feb 1, 2014·Genome Announcements·Nagaraja R ThirumalapuraDavid H Walker
Jan 30, 2016·Vector Borne and Zoonotic Diseases·Sandor E KarpathyMichael L Levin
Jun 6, 2009·Parasite Immunology·S ThomasD H Walker
Apr 1, 2015·Seminars in Immunopathology·David H Walker, J Stephen Dumler
Jul 6, 2014·The Journal of Infectious Diseases·Tais Berelli SaitoDavid H Walker
Jun 16, 2016·Frontiers in Cellular and Infection Microbiology·Taslima T LinaJere W McBride
May 3, 2005·Current Opinion in Infectious Diseases·Gary M Winslow, Constantine Bitsaktsis
Feb 17, 2006·Annals of the New York Academy of Sciences·Michael E WoodsJuan P Olano
Feb 17, 2006·Annals of the New York Academy of Sciences·Patricia A Crocquet-ValdesDavid H Walker
Feb 18, 2010·Expert Review of Clinical Immunology·Javid P Mohammed, Jochen Mattner
May 12, 2019·Applied and Environmental Microbiology·Geoffrey E LynnUlrike G Munderloh
Dec 15, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katherine C MacNamaraGary M Winslow
Jul 1, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Purnima GhoseNahed Ismail
Sep 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Constantine Bitsaktsis, Gary Winslow
Aug 31, 2016·Veterinary Sciences·Tais B Saito, David H Walker
May 28, 2019·Frontiers in Immunology·Tyler R TominelloNahed Ismail
Jul 9, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rachael RacineGary M Winslow

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