Histological analysis of microencapsulated dopamine-secreting cells in agarose/poly(styrene sulfonic acid) mixed gel xenotransplanted into the brain

Experimental Neurology
Y MiyoshiHiroo Iwata

Abstract

PC12 cells were encapsulated within agarose/poly-(styrene sulfonic acid) (agarose/PSSa) mixed gel and xenotransplanted into the brains of guinea pigs. The immunoprotection capability and biocompatibility of the agarose/PSSa microcapsules were evaluated. Tyrosine hydroxylase-positive PC12 cells within the microcapsules were observed in all guinea pigs at least 5 weeks after transplantation. PC12 cells had a relatively uniform small size and occasionally formed cell clusters; however, cell necrosis was not apparent. The host reaction to agarose/PSSa microcapsules was minimal. The degree of glial fibrillary acidic protein-positive astrocyte density around the microcapsules was similar to that around the injection tracks. Necrosis around the brain/microcapsule interface was not apparent as assessed by Nissl staining. Normal-appearing neurons were observed in close vicinity to the capsule. High-performance liquid chromatography with electrochemical detection showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. We conclude that encapsulation using agarose/PSSa mixed gel can not only isolate the enclosed cells from the host immune system but can also allow diffusional exchange of nu...Continue Reading

Citations

Oct 15, 1998·The New England Journal of Medicine·A E Lang, A M Lozano
Jan 31, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·M P ZaninR K Shepherd
Jun 25, 2009·Biotechnology Progress·Jean-Michel RabanelPatrice Hildgen
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May 10, 2018·Cell Transplantation·Yu-Shuan ChenTzyy-Wen Chiou
Jan 5, 2006·Journal of Biomedical Materials Research. Part a·Kenta MoriyasuHiroo Iwata
Sep 9, 1999·Cell Transplantation·H YoshidaT Ohmoto

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