Histological and molecular confirmation of the fourth human case caused by Dirofilaria repens in a new endemic region of Slovakia

Journal of Helminthology
G HrčkovaS Kovacs

Abstract

Dirofilariosis is considered to be the arthropod vector-borne disease with the fastest spread in Europe. Slovakia belongs to new endemic regions for canine and human infections. This paper reports the fourth human case, where diagnosis was confirmed using computed tomography (CT), histological and molecular examinations - for the first time in this endemic region. The epidemiological history of this case indicated the autochthonous origin, and infection manifested by intense swelling in the periocular region, where a subcutaneous nodule was localized. Microscopic analysis of cross-sections of the surgically removed nodule confirmed the presence of a single male worm of Dirofilaria repens, indicated by the external cuticular ridges, the robust muscle cells and a single male sex organ seen as one tube beside the intestine. Considering that the worm morphology was partially damaged, molecular study was performed using DNA isolated from formalin-fixed and paraffin-embedded tissue sections of the worm. In polymerase chain reactions (PCR) a set of primers specific for D. repens DNA for the CO1-encoding gene amplified the expected 246 bp product using a high concentration of DNA template. Our diagnostic approach, involving molecular t...Continue Reading

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Citations

Aug 24, 2012·Journal of Clinical Microbiology·Kelvin K W ToKwok-Yung Yuen
Oct 28, 2014·Parasitology Research·Ivo RudolfZdeněk Hubálek
Mar 30, 2016·Parasitology Research·Martina MiterpákováDana Miklisová
May 3, 2015·Parasitology Research·Daniela AntolováZuzana Paraličová
Apr 25, 2013·Journal of Abnormal Child Psychology·Paul A Boelen, Mariken Spuij
Oct 2, 2019·Vestnik oftalmologii·M B GushchinaN S Yuzhakova
Dec 21, 2018·Parasites & Vectors·Gioia CapelliCornelia Silaghi
Jul 25, 2017·Wiener klinische Wochenschrift·Martina MiterpákováViliam Gál

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