Histological and SEM analysis of root cementum following irradiation with Er:YAG and CO2 lasers.

Lasers in Medical Science
Aslam AlmehdiYuichi Izumi

Abstract

Recently, the Er:YAG and CO(2) lasers have been applied in periodontal therapy. However, the characteristics of laser-irradiated root cementum have not been fully analyzed. The aim of this study was to precisely analyze the alterations of root cementum treated with the Er:YAG and the CO(2) lasers, using non-decalcified thin histological sections. Eleven cementum plates were prepared from extracted human teeth. Pulsed Er:YAG laser contact irradiation was performed in a line at 40 mJ/pulse (14.2 J/cm(2)/pulse) and 25 Hz (1.0 W) under water spray. Continuous CO(2) laser irradiation was performed in non-contact mode at 1.0 W, and ultrasonic instrumentation was performed as a control. The treated samples were subjected to stereomicroscopy, scanning electron microscopy (SEM), light microscopy and SEM energy dispersive X-ray spectroscopy (SEM-EDS). The Er:YAG laser-treated cementum showed minimal alteration with a whitish, slightly ablated surface, whereas CO(2) laser treatment resulted in distinct carbonization. SEM analysis revealed characteristic micro-irregularities of the Er:YAG-lased surface and the melted, resolidified appearance surrounded by major and microcracks of the CO(2)-lased surface. Histological analysis revealed mini...Continue Reading

References

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Citations

Apr 14, 2015·Periodontology 2000·Akira AokiYuichi Izumi
Mar 30, 2016·Lasers in Surgery and Medicine·Taichen LinYuichi Izumi
Aug 14, 2015·Photomedicine and Laser Surgery·Jutipond PhanombualertWilawan Weera-Archakul
Jan 3, 2015·Photomedicine and Laser Surgery·Mary A S MeloLidiany K A Rodrigues
Feb 26, 2015·The Open Dentistry Journal·Vamsi LavuSuresh Ranga Rao
Mar 31, 2017·The Open Dentistry Journal·Joseph D EverettIbtisam Al-Hashimi
May 6, 2019·Photobiomodulation, Photomedicine, and Laser Surgery·Muftah Agoob AlferganyNorbert Gutknecht

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