Histological changes of the testis and epididymis in adult rats as a result of Leydig cell destruction after ethane dimethane sulfonate treatment: a morphometric study

Asian Journal of Andrology
Zheng-Wei YangNathaniel Mills

Abstract

To quantitatively study the histological changes of the testis and epididymis as a result of a drastic reduction of testosterone secretion. Fourteen adult Sprague-Dawley rats were injected intraperitoneally with ethane dimethane sulfonate (EDS, 75 mg/kg) and the same number of animals were injected with normal saline as a control. At days 7 and 12 (after treatment), respectively, half of the animals from each group were killed. The testes and epididymides were removed and tissue blocks embedded in methacrylate resin. The cell number per testis was estimated using the stereological optical disector and some other parameters were obtained using other morphometric methods. The EDS treatment resulted in an almost complete elimination of Leydig cells but had no effect on the numbers of Sertoli cells per testis. At day 7 after EDS treatment, many elongated spermatids were retained in the seminiferous epithelium and many round spermatids could be seen in the epididymal ducts. At day 12, a looser arrangement of spermatids and spermatocytes became evident, with apparent narrow empty spaces being formed between germ cells in an approximately radial direction towards the tubule lumen; the numbers (per testis) of non-type B spermatogonia a...Continue Reading

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Citations

Mar 2, 2013·International Journal of Environmental Research and Public Health·Mukhtar AhmedTajamul Hussain
Dec 22, 2007·Asian Journal of Andrology·Imen HammamiMichele Veronique El May
Dec 22, 2007·Asian Journal of Andrology·Deivendran RengarajZeng-Ming Yang
Apr 4, 2008·Asian Journal of Andrology·Bin PengZheng-Wei Yang
Mar 21, 2007·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Bharati G KhobarekarAtmaram H Bandivdekar
Sep 15, 2010·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Qing ChangChengxia Zhang
Jun 20, 2012·Journal of Biochemical and Molecular Toxicology·Eun-Hee LeeSeokjoo Yoon
Sep 18, 2018·Journal of Behavioral Addictions·Ágnes ZsilaZsolt Demetrovics
May 31, 2014·Trauma, Violence & Abuse·Joanne Belknap, Nitika Sharma
Feb 13, 2020·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Khaled HabasDiana Anderson
May 1, 2014·Spermatogenesis·Wilma De Grava Kempinas, Gary Robert Klinefelter
Nov 13, 2014·American Journal of Physiology. Endocrinology and Metabolism·M V T LoboA Martín-Hidalgo
Feb 22, 2011·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Sherin W Abdelmalik

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