Histological effect of nicotine on adrenal zona fasciculata and the effect of grape seed extract with or without withdrawal of nicotine

Journal of Microscopy and Ultrastructure
Hanaa A KhalafEl-Hassanen M Mahmoud

Abstract

Cigarette smoking is harmful to the health of both smokers and nonsmokers. It is a major cause of death. This study aimed to investigate the structural changes in the zona fasciculata of albino rats caused by nicotine and the protective effect of grape seeds with or without the stoppage of nicotine administration. Thirty-five adult male rats were used and equally divided into five groups: negative and positive control groups (Groups I and II), nicotine-treated group (Group III), nicotine- and grape seed extract-treated group (Group IV), and nicotine withdrawal and grape seed extract-treated group (Group V). Adrenal glands were dissected and prepared for histological studies. The majority of zona fasciculata cells of Group III showed striking changes in terms of swelling of the cells with marked cytoplasmic vacuolation, many pyknotic nuclei, and increased immunoexpression to caspase 3 antibodies. By electron microscopy, a marked increase in lipid deposition with its appearance in the capillary between zona fasciculata cells was noticed. Heterochromatic nuclei and dilated smooth endoplasmic reticulum were noted. Degenerated mitochondria and some mitochondria that had cavitation with a progressive loss of their cristae were seen. ...Continue Reading

References

Aug 3, 2004·Science·Douglas R Green, Guido Kroemer
Feb 11, 2005·American Journal of Physiology. Renal Physiology·Ganesan Ramesh, W Brian Reeves
Mar 22, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Alan Shihadeh, Rawad Saleh
Apr 9, 2005·European Journal of Endocrinology·D Kapoor, T H Jones
Apr 16, 2005·Fertility and Sterility·Khae-Hawn KimChang-Ju Kim
Sep 16, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Gabriel PerlemuterCatherine Buffet
Oct 6, 2006·Molecular and Cellular Biochemistry·Johnny BarrGovindarajan T Ramesh
Jun 1, 2007·Applied Immunohistochemistry & Molecular Morphology : AIMM·Bettine A H VossePu Yan
Sep 12, 2007·Apoptosis : an International Journal on Programmed Cell Death·Reinhard ZeidlerStephan Lang
Nov 15, 2007·Synapse·Mario SabhaAlexandre Leite Rodrigues De Oliveira
May 9, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M I YousefL K El-Shennawy
Nov 6, 2010·Endocrine Reviews·Walter L Miller, Richard J Auchus
Apr 19, 2011·Archives of Oral Biology·S W KangJ O Cho
Jul 1, 2011·Andrologia·W H NesseimT Mostafa
Aug 22, 2013·Molecular Nutrition & Food Research·Hui WangMei-Jun Zhu
Dec 19, 2013·Experimental and Therapeutic Medicine·Jiangong ZhangZengli Yu
Dec 5, 2014·International Journal of Stem Cells·Zeinab Mohamed Kamel IsmailAlshaymaa Gamal Aboulkhair
Mar 27, 2015·Indian Journal of Medical and Paediatric Oncology : Official Journal of Indian Society of Medical & Paediatric Oncology·Aseem MishraApurva Garg
Aug 14, 2015·Cleveland Clinic Journal of Medicine·Jason M JerryDavid Streem
Dec 20, 2015·BMC Complementary and Alternative Medicine·Nurhanani RazaliAzlina Abdul Aziz
Jan 30, 2016·Oxidative Medicine and Cellular Longevity·Zhiqi WangDeliang Cao

❮ Previous
Next ❯

Citations

May 6, 2020·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amany Mohamed ShalabyDina Fouad El Shaer

❮ Previous
Next ❯

Methods Mentioned

BETA
transmission electron microscopy
MDA

Software Mentioned

Video Test Morphology

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.