Histological Scoring Method to Assess Bone Healing in Critical Size Bone Defect Models

Tissue Engineering. Part C, Methods
Zhihua HanJohn H Barker

Abstract

Large bone defects are a major problem in trauma and orthopedic surgery. Tissue engineering based treatments have emerged as promising alternatives to traditional bone grafting techniques. Critical size bone defect animal models have been developed and widely used to evaluate and compare therapeutic effectiveness in bone tissue engineering treatments. To measure healing in a given defect after treatment, histological assessment methods are commonly used. These histological methods are typically qualitative and only measure the amount of newly formed bone. In this study, we introduce a new histological scoring method that in addition to new bone formation also measures newly formed "cartilage," "fibrous tissue," and "remnant bone defect size." Using Kappa analysis and interclass correlation analysis, we verified the reliability of our new scoring method. These additional parameters make it possible to differentiate between the hard callus and soft callus phases of healing and, thus, derive more valuable information about the effect different tissue-engineering treatments have on the healing process.

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Citations

Jul 7, 2019·Journal of Tissue Engineering and Regenerative Medicine·Chau Sang LauSwee-Hin Teoh
Sep 3, 2019·Journal of Tissue Engineering and Regenerative Medicine·Ahmet Engin PazarçevirenAyşen Tezcaner
Dec 13, 2019·Stem Cell Reviews and Reports·Liudmila LeppikJ H Barker
Jul 31, 2020·Tissue Engineering. Part C, Methods·Flavia Medeiros SaviLaura Jane Bray
Jul 11, 2020·Journal of the Chinese Medical Association : JCMA·Jir-You Wang, Cheng-Fong Chen
Dec 9, 2020·Clinical and Experimental Dental Research·Yuan-Wu ChenHua-Hong Chien
Apr 10, 2020·Current Stem Cell Research & Therapy·Weidong ZhangFengxuan Han
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Mahmut ParmaksizYaşar Murat Elçin
May 25, 2021·Journal of Advanced Research·Lan LiQing Jiang
Aug 27, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Adam M KnoxChristopher D Collier

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Methods Mentioned

BETA
light microscopy

Software Mentioned

GraphPad Prism
KEYENCE ANALYZER
SPSS

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