PMID: 6160210Jun 1, 1980Paper

Histological staining of pre- and postsynaptic components of amphibian neuromuscular junctions

Journal of Neurocytology
M S Letinsky, P A Decino

Abstract

We have developed a combined staining technique whereby pre- and postsynaptic structures of the neuromuscular junction can be simultaneously visualized in the light microscope. The general approach was first to stain presynaptic nerve terminals using nitroblue tetrazolium (NBT), which when reduced to its diformazan state coloured the entire nerve terminal arborization blue. When the NBT stain was combined with the Karnovsky acetylcholinesterase (AChE) procedure the blue-coloured nerve terminal processes were vividly outlined by the brown copper ferrocyanide-AChE reaction product. Experiments were performed to ensure that the NBT AChE method stained nerve reliably and that even extremely small neural processes were stained and visualized in the light microscope. This method stained cholinergic neuromuscular junctions and unmyelinated axons in a variety of preparations. Aldehyde pre-and poststaining fixation markedly affected the quality of NBT nerve stain. In addition, glutaraldehyde had a direct role in the staining process. The quality of the nerve terminal staining was affected by the pH and the constituents of the staining solution. The powerful experimental advantage of the NBT-AChE stain for neuromuscular junctions resulte...Continue Reading

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Citations

Feb 1, 1985·Journal of Neurocytology·A A Herrera, D R Scott
Jun 1, 1980·Journal of Neurocytology·M S Letinsky, K Morrison-Graham
Dec 1, 1985·Journal of Neurocytology·M R BennettN A Lavidis
Oct 1, 1983·Developmental Biology·K Morrison-Graham
Jun 1, 1986·Journal of Neuroscience Methods·R Butler, I M Payk
Feb 1, 1984·Brain Research·A E SchaffnerM C Fishman
Jan 1, 1991·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·N Oren, M S Letinsky
May 1, 1995·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·M KawabuchiK Hirata
Mar 1, 1988·Experimental Neurology·D C Linden, M S Letinsky
Mar 1, 1988·Experimental Neurology·D C LindenM S Letinsky
Mar 14, 1998·Developmental Biology·A Dunaevsky, E A Connor
Jan 1, 1990·Journal of Neurobiology·A A Herrera, M J Werle
Sep 22, 1989·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P A Pawson, A D Grinnell
Jun 1, 1988·Journal of Neurobiology·C L JordanA P Arnold
Dec 1, 1996·Journal of Neurobiology·A D Grinnell, Y Harada
Sep 1, 1985·Muscle & Nerve·J S Gunther, M S Letinsky

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