Histological validation of in vivo assessment of cancer tissue inhomogeneity and automated morphological segmentation enabled by Optical Coherence Elastography.

Scientific Reports
Anton A PlekhanovVladimir Y Zaitsev

Abstract

We present a non-invasive (albeit contact) method based on Optical Coherence Elastography (OCE) enabling the in vivo segmentation of morphological tissue constituents, in particular, monitoring of morphological alterations during both tumor development and its response to therapies. The method uses compressional OCE to reconstruct tissue stiffness map as the first step. Then the OCE-image is divided into regions, for which the Young's modulus (stiffness) falls in specific ranges corresponding to the morphological constituents to be discriminated. These stiffness ranges (characteristic "stiffness spectra") are initially determined by careful comparison of the "gold-standard" histological data and the OCE-based stiffness map for the corresponding tissue regions. After such pre-calibration, the results of morphological segmentation of OCE-images demonstrate a striking similarity with the histological results in terms of percentage of the segmented zones. To validate the sensitivity of the OCE-method and demonstrate its high correlation with conventional histological segmentation we present results obtained in vivo on a murine model of breast cancer in comparative experimental study of the efficacy of two antitumor chemotherapeutic...Continue Reading

References

Jan 1, 1989·Cancer Chemotherapy and Pharmacology·M M Tomayko, C P Reynolds
Sep 2, 1995·BMJ : British Medical Journal·E EypaschH Troidl
Apr 10, 1999·Ultrasonic Imaging·T A KrouskopT Hall
Mar 25, 2005·Nature Reviews. Drug Discovery·Dong Wang, Stephen J Lippard
Jul 22, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Thomas FriessMax Hasmann
Apr 25, 2008·Current Protocols in Immunology·B A Pulaski, S Ostrand-Rosenberg
May 8, 2009·Physics in Medicine and Biology·Steven G AdieDavid D Sampson
Dec 10, 2009·Optics Express·Brendan F KennedyDavid D Sampson
Dec 2, 2010·Physics in Medicine and Biology·K J ParkerD J Rubens
Jan 26, 2011·Optics Express·Angelo PierangeloAntonello De Martino
Jun 18, 2011·Breast Cancer Research and Treatment·Jung Min ChangSeung Ja Kim
Aug 10, 2012·Biomedical Optics Express·Brendan F KennedyDavid D Sampson
Jan 22, 2013·International Journal of Cancer. Journal International Du Cancer·Sandra HeskampHanneke W M van Laarhoven
Nov 14, 2013·Journal of Biomedical Optics·Kelsey M KennedyDavid D Sampson
Nov 18, 2014·Biomedical Optics Express·Lixin ChinDavid D Sampson
Nov 9, 2016·Journal of Biomedical Optics·Vladimir Y ZaitsevAlex Vitkin
Nov 15, 2016·Seminars in Cancer Biology·Duojiao WuXiangdong Wang
Feb 17, 2017·Journal of Biomedical Optics·Ivan A BratchenkoValery P Zakharov
Nov 8, 2017·Biochemical and Biophysical Research Communications·Taichi IkedoSusumu Miyamoto
Dec 6, 2017·Scientific Reports·Peter BankheadPeter W Hamilton
Mar 20, 2018·Biomedical Optics Express·Hinnerk Schulz-HildebrandtGereon Hüttmann
Apr 18, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rodrigo A NatalLuís Otávio Sarian
Feb 26, 2019·Biomedical Optics Express·Li DongAssad A Oberai
Mar 21, 2019·Biomedical Optics Express·Matt S HepburnBrendan F Kennedy
May 31, 2019·Nature Biomedical Engineering·Yair RivensonAydogan Ozcan

❮ Previous
Next ❯

Citations

Oct 1, 2020·Scientific Reports·Rowan W SandersonBrendan F Kennedy
Aug 24, 2021·Ultraschall in der Medizin : Organ der Deutschen Gesellschaft für Ultraschall in der Medizin, [der] Österreichischen Gesellschaft für Ultraschall in der Medizin, [der] Schweizerischen Gesellschaft für Ultraschall in Medizin und Biologie·Michael GolattaRichard G Barr

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy

Software Mentioned

QuPath
nulldelimiterspace
Statistica

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.