Histomorphologic characterization of noncarious and caries-affected dentin/adhesive interfaces

Journal of Prosthodontics : Official Journal of the American College of Prosthodontists
Reem Haj-AliPaulette Spencer

Abstract

The purpose of this study was to compare the dentin/adhesive interfacial characteristics when bonding to noncarious as well as caries-affected dentin. Seven extracted, unerupted, third molars were sectioned into halves. Artificial caries was created on one-half of each tooth, leaving the other half as a control. Dentin surfaces were treated with UNO adhesive according to the manufacturer's instructions for the wet-bonding technique and under environmental conditions present in the oral cavity. Dentin/adhesive interface sections of each half-tooth were stained with Goldner's trichrome, a classic bone stain, and examined using light microscopy. The width of exposed collagen was measured directly from photomicrographs, and adhesive penetration was analyzed qualitatively. The degree and extent to which the adhesive encapsulated the demineralized dentin matrix were reflected in the color difference in the stained sections with the noncarious dentin sections showing a degree of collagen encapsulation superior to that of the caries-affected dentin sections. The overall mean widths of exposed collagen were significantly (p < or = .05) greater at the caries-affected dentin/adhesive interface, 8.6 (1.7) microm, as compared with those at ...Continue Reading

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Citations

Mar 3, 2010·Annals of Biomedical Engineering·Paulette SpencerJ Lawrence Katz
May 30, 2012·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Manuel ToledanoRaquel Osorio
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Mar 1, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·Yan LiuFranklin R Tay
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