Histone acetyltransferase KAT8 is essential for mouse oocyte development by regulating reactive oxygen species levels.

Development
Shi YinQinghua Shi

Abstract

Proper oocyte development is crucial for female fertility and requires timely and accurate control of gene expression. K (lysine) acetyltransferase 8 (KAT8), an important component of the X chromosome dosage compensation system in Drosophila, regulates gene activity by acetylating histone H4 preferentially at lysine 16. To explore the function of KAT8 during mouse oocyte development, we crossed Kat8flox/flox mice with Gdf9-Cre mice to specifically delete Kat8 in oocytes. Oocyte Kat8 deletion resulted in female infertility, with follicle development failure in the secondary and preantral follicle stages. RNA-seq analysis revealed that Kat8 deficiency in oocytes results in significant downregulation of antioxidant genes, with a consequent increase in reactive oxygen species. Intraperitoneal injection of the antioxidant N-acetylcysteine rescued defective follicle and oocyte development resulting from Kat8 deficiency. Chromatin immunoprecipitation assays indicated that KAT8 regulates antioxidant gene expression by direct binding to promoter regions. Taken together, our findings demonstrate that KAT8 is essential for female fertility by regulating antioxidant gene expression and identify KAT8 as the first histone acetyltransferase w...Continue Reading

References

Oct 6, 2001·Biochemical and Biophysical Research Communications·P SvobodaR M Schultz
Dec 18, 2001·Genesis : the Journal of Genetics and Development·C YanM M Matzuk
Oct 9, 2002·Nature Genetics·Soazik P JaminRichard R Behringer
Mar 30, 2005·Proceedings of the National Academy of Sciences of the United States of America·Michael F DionOliver J Rando
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Kenneth P McNattyMika P E Laitinen
Jan 25, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Shun-ichiro KageyamaFugaku Aoki
Mar 17, 2007·Annual Review of Biochemistry·Mona D Shahbazian, Michael Grunstein
Apr 21, 2007·BMC Developmental Biology·Diana LuciferoJacquetta M Trasler
Jun 26, 2007·The Annals of Thoracic Surgery·Ilhan InciWalter Weder
May 3, 2008·Journal of Cellular Physiology·P RodriguesD F Albertini
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Aug 7, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ruth A RobertsWilliam Slikker
May 19, 2010·Molecular and Cellular Biology·Girdhar G SharmaTej K Pandita
Feb 16, 2011·Proceedings of the National Academy of Sciences of the United States of America·Rakesh KumarTej K Pandita
Jun 23, 2011·Transplantation Proceedings·A DanilovicA C Seguro
Aug 23, 2011·Molecular and Cellular Endocrinology·Krishna Jagarlamudi, Aleksandar Rajkovic
Oct 29, 2011·Biology of Reproduction·Patrick J DevineUlrike Luderer
Jan 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Pengpeng MaRichard M Schultz
Apr 3, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Zengxiang PanHonglin Liu
Oct 10, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sang Bum KimJerry W Shay
Dec 4, 2012·Biochimica Et Biophysica Acta·Regina Brigelius-Flohé, Matilde Maiorino
Dec 3, 2014·Reproductive Biomedicine Online·Monireh MahmoodiAbdulhussein Shahverdi
May 11, 2015·Apoptosis : an International Journal on Programmed Cell Death·Meenakshi TiwariShail K Chaube

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Citations

Sep 8, 2018·Journal of Cellular Biochemistry·Lei-Lei YangShen Yin
Mar 29, 2019·Science China. Life Sciences·Zhu HuangQinghua Shi
Apr 18, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yumeng CaoRan Huo
Dec 4, 2019·The Journal of Clinical Investigation·Lin LiXiang-Jiao Yang
Feb 27, 2021·Journal of Cellular Physiology·Meng WangXiangzhi Li
Feb 23, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jasmine A Burrell, Jacqueline M Stephens
Apr 13, 2021·Frontiers in Cell and Developmental Biology·Meina HeChao Wang

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