Histone chaperone Rtt106 promotes nucleosome formation using (H3-H4)2 tetramers.

The Journal of Biological Chemistry
A M FazlyZhiguo Zhang

Abstract

The yeast histone chaperone Rtt106 is involved in de novo assembly of newly synthesized histones into nucleosomes during DNA replication and plays a role in regulating heterochromatin silencing and maintaining genomic integrity. The interaction of Rtt106 with H3-H4 is modulated by acetylation of H3 lysine 56 catalyzed by the lysine acetyltransferase Rtt109. Using affinity purification strategies, we demonstrate that Rtt106 interacts with (H3-H4)(2) heterotetramers in vivo. In addition, we show that Rtt106 undergoes homo-oligomerization in vivo and in vitro, and mutations in the N-terminal homodimeric domain of Rtt106 that affect formation of Rtt106 oligomers compromise the function of Rtt106 in transcriptional silencing and response to genotoxic stress and the ability of Rtt106 to bind (H3-H4)(2). These results indicate that Rtt106 deposits H3-H4 heterotetramers onto DNA and provide the first description of a H3-H4 chaperone binding to (H3-H4)(2) heterotetramers in vivo.

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Citations

Jan 5, 2013·Nature Structural & Molecular Biology·Rebecca J Burgess, Zhiguo Zhang
Sep 4, 2012·Nucleic Acids Research·Duane D WinklerKarolin Luger
Aug 27, 2013·Cellular and Molecular Life Sciences : CMLS·Christoph F KuratJeffrey Fillingham
Jan 26, 2013·Current Opinion in Genetics & Development·Iestyn Whitehouse, Duncan J Smith
Jan 6, 2017·Nature Reviews. Molecular Cell Biology·Colin M HammondAnja Groth
Feb 15, 2018·Proceedings of the National Academy of Sciences of the United States of America·Ryan JankeJasper Rine
May 17, 2018·The Journal of Biological Chemistry·Gabriele MarcianòDanny T Huang
Mar 17, 2019·Bioscience Reports·Callum J O'Kane, Edel M Hyland
Dec 8, 2017·Oncotarget·Qianyun MeiShanshan Li
Mar 20, 2020·Cell & Bioscience·Wenshuo ZhangQing Li
May 2, 2019·The Journal of Biological Chemistry·M Daniel RickettsRonen Marmorstein
Jul 12, 2017·Frontiers in Genetics·Hollie RowlandsKrassimir Yankulov
Dec 19, 2019·Nature Communications·Carol ChoJi-Joon Song
Jun 5, 2021·DNA Repair·Ian Hammond-MartelHugo Wurtele
Nov 15, 2013·Epigenetics & Chromatin·Varija N BudhavarapuJessica K Tyler

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