Histone code modifications on pluripotential nuclei of reprogrammed somatic cells.

Molecular and Cellular Biology
Hironobu KimuraTakashi Tada

Abstract

Following hybridization with embryonic stem (ES) cells, somatic genomes are epigenetically reprogrammed and acquire pluripotency. This results in the transcription of somatic genome-derived tissue-specific genes upon differentiation. During nuclear reprogramming, it is expected that DNA and chromatin modifications, believed to function in cell-type-specific epigenotype memory, should be significantly modified. Indeed, current evidence indicates that acetylation and methylation of histone H3 and H4 amino termini play a major role in the regulation of gene activity through the modulation of chromatin conformation. Here, we show that the reprogrammed somatic genome of ES hybrid cells becomes hyperacetylated at H3 and H4, while lysine 4 (K4) of H3 becomes globally hyper-di- and -tri-methylated. In the Oct4 promoter region, histones H3 and H4 are acetylated and H3-K4 is highly tri-methylated on both the ES and reprogrammed somatic genomes, which correlates with gene activation and DNA demethylation. However, H3-K4 is also di- and tri-methylated in the promoter regions of Neurofilament-M (Nfm), Nfl, and Thy-1, which are all silent in both ES and hybrid cells. Thus, H3-K4 di- and tri-methylation of reprogrammed somatic genomes is inde...Continue Reading

References

Mar 28, 1998·Trends in Biochemical Sciences·B R Cairns
Jun 4, 1998·Current Opinion in Genetics & Development·J A Armstrong, B M Emerson
Jun 26, 1998·Current Opinion in Cell Biology·P D Varga-Weisz, P B Becker
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Sep 29, 1999·Genes & Development·R E Kingston, G J Narlikar
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D StrahlC D Allis
Feb 2, 2000·Nature Genetics·W M RideoutR Jaenisch
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·E C ForsbergE H Bresnick
Mar 17, 2001·Nature Reviews. Genetics·D Solter
Mar 27, 2001·Current Biology : CB·C J Fry, C L Peterson
Mar 29, 2001·Trends in Genetics : TIG·F Lyko
Mar 30, 2001·Nature Genetics·E Whitelaw, D I Martin
Jun 8, 2001·Molecular and Cellular Biology·D O'CarrollT Jenuwein
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·W DeanW Reik
Mar 19, 2002·Nature·James P JacksonSteven E Jacobsen
Apr 9, 2002·Genes & Development·Junji FujikuraHitoshi Niwa
May 23, 2002·Genes & Development·Michele BoianiK John McLaughlin
Jul 12, 2002·The Journal of Biological Chemistry·Sharon Gidekel, Yehudit Bergman
Aug 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ken-ichi Noma, Shiv I S Grewal
Sep 28, 2002·Nature·Helena Santos-RosaTony Kouzarides
Nov 28, 2002·Molecular Cell·Thomas A MilneJay L Hess
Mar 22, 2003·Science·Kathrin PlathYi Zhang
May 6, 2003·Journal of Cell Science·Monika LachnerThomas Jenuwein
Jul 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masako TadaTakashi Tada

❮ Previous
Next ❯

Citations

Nov 16, 2007·Cell and Tissue Research·Stuart Atkinson, Lyle Armstrong
Mar 26, 2005·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Fabio SpadaEric M Thompson
Nov 1, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Musashi KubiuraMasako Tada
Feb 13, 2008·In Vitro Cellular & Developmental Biology. Animal·Gen-Bao ShaoAi-Hua Gong
Mar 26, 2010·Stem Cell Reviews and Reports·Minal Patel, Shuying Yang
Feb 7, 2008·Reproductive Biomedicine Online·Hiroyuki Matsumura, Takashi Tada
Jun 4, 2013·Acta Pharmacologica Sinica·Jian-bin SuBao-ming Qin
Jun 11, 2010·Nature·Shinya Yamanaka, Helen M Blau
Oct 2, 2004·Nature Cell Biology·Naoyuki Fujita, Paul A Wade
Jan 18, 2006·Nature Cell Biology·Nirit FeldmanYehudit Bergman
May 26, 2006·Nature Reviews. Molecular Cell Biology·Eran Meshorer, Tom Misteli
Jun 24, 2011·Nature Reviews. Molecular Cell Biology·Jerome JullienJ B Gurdon
Nov 7, 2007·Nature Structural & Molecular Biology·Sukesh R BhaumikAli Shilatifard
Mar 11, 2008·Proceedings of the National Academy of Sciences of the United States of America·Doug PhanstielJoshua J Coon
Jan 27, 2005·Cloning and Stem Cells·Ian Chambers
Mar 28, 2007·Cloning and Stem Cells·Philippe Collas, Runhild Gammelsaeter
Oct 3, 2006·Cloning and Stem Cells·Stephen SullivanJim McWhir
Jul 23, 2009·Cloning and Stem Cells·Raymond L PageTanja Dominko
Sep 6, 2007·Stem Cells and Development·Rishi Sharma, Ram Raghubir
Feb 23, 2007·Molecular Biology of the Cell·Christel T FrebergPhilippe Collas
Jul 18, 2008·Human Molecular Genetics·Victoria V Lunyak, Michael G Rosenfeld
Oct 12, 2010·Molecular Human Reproduction·Tatjana KhromovUlrich Zechner
Apr 1, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Shinya Yamanaka
Jul 6, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Francesco M PiccoloAmanda G Fisher
Nov 5, 2010·Cold Spring Harbor Symposia on Quantitative Biology·V PasqueJ B Gurdon
Mar 20, 2010·Development, Growth & Differentiation·Kazutoshi Takahashi
Mar 4, 2005·Molecular and Cellular Biology·Takao KurodaTakashi Tada
Oct 1, 2008·Tsitologiia·A G MenzorovO L Serov
May 18, 2006·Annual Review of Cell and Developmental Biology·J B Gurdon
Aug 16, 2006·Neuro-degenerative Diseases·Takashi Tada
Jan 1, 2008·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Philippe CollasAnita L Sørensen
Apr 15, 2008·The Journal of Reproduction and Development·Gen-Bao ShaoDe-Sheng Xiao
Jul 14, 2011·Regenerative Medicine·Cédric BoucherieRobin R Ali
Jan 25, 2012·International Journal of Molecular Sciences·Shahram GolbabapourMaryam Hajrezaei
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Ramiro AlberioAndrew D Johnson
Aug 31, 2013·Rejuvenation Research·Rong XuAnmin Lei
May 3, 2014·Cellular Reprogramming·Huseyin SumerPaul J Verma

❮ Previous
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