Histone deacetylase inhibitors improve in vitro and in vivo developmental competence of somatic cell nuclear transfer porcine embryos.

Cellular Reprogramming
Jianguo ZhaoR S Prather

Abstract

Faulty epigenetic reprogramming of somatic nuclei is likely to be a major cause of low success observed in all mammals produced through somatic cell nuclear transfer (SCNT). It has been demonstrated that the developmental competence of SCNT embryos in several species were significantly enhanced via treatment of histone deacetylase inhibitors (HDACi) such as trichostatin A (TSA) to increase histone acetylation. Here we report that 50 nM TSA for 10 h after activation increased the developmental competence of porcine SCNT embryos constructed from Landrace fetal fibroblast cells (FFCs) in vitro and in vivo, but not at higher concentrations. Therefore, we optimized the application of another novel HDACi, Scriptaid, for development of porcine SCNT embryos. We found that treatment with 500 nM Scriptaid significantly enhanced the development SCNT embryos to the blastocyst stage when outbred Landrace FFCs and ear fibroblast cells (EFCs) were used as donors compared to the untreated group. Scriptaid increased the overall cloning efficiency from 0.4% (untreated group) to 1.6% for Landrace FFCs and 0 to 3.7% for Landrace EFCs. Moreover, treatment of SCNT embryos with Scriptaid improved the histone acetylation on Histone H4 at lysine 8 (AcH...Continue Reading

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Citations

Jun 22, 2010·Cell and Tissue Research·Jianguo ZhaoRandall S Prather
Oct 25, 2011·Journal of Assisted Reproduction and Genetics·Shahram JafariMohammad Hosein Nasr-Esfahani
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Jul 2, 2011·Molecular Reproduction and Development·Shahram JafariMohammad H Nasr-Esfahani
Nov 26, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Jiaojiao HuangJianguo Zhao
Jul 23, 2014·Asian-Australasian Journal of Animal Sciences·Yeon Ik JeongWoo Suk Hwang
Jul 23, 2014·The Journal of Reproduction and Development·Yan Jun HuanHong Bin He
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Dec 31, 2014·Cellular Reprogramming·Jiude MaoRandall S Prather
Jun 14, 2013·Zygote : the Biology of Gametes and Early Embryos·Xian-Rong XiongJin-Cheng Zhong

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Methods Mentioned

BETA
cesarean section
acetylation
histone acetylation
ubiquitination

Software Mentioned

SAS
Nikon NIS element

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