Histone H1 quantity determines the efficiencies of apoptotic DNA fragmentation and chromatin condensation

Biomedical Research
Marie Kijima, Ryushin Mizuta

Abstract

Oligonucleosomal DNA fragmentation and chromatin condensation are two hallmarks of apoptosis. However, their generation mechanisms are not entirely understood. Histone H1, a positively charged nuclear protein located in the linker region of chromatin, is involved in higher-order chromatin structures and tight chromatin packing. On the basis of the physical and biochemical characteristics of histone H1, we hypothesized that histone H1 plays a role in determining the efficiencies of apoptotic DNA fragmentation and chromatin condensation. Therefore, we examined histone H1 quantity in five human leukemia cell lines and compared the efficiencies. The cell lines were categorized into two groups according to their origins: (i) Ramos and Molt-4 cells of lymphoid origin and (ii) U937, ML-1, and HL60 cells of myeloid origin. Compared to the lymphoid-origin group, the myeloid-origin group had lower levels of histone H1 but more open chromatin. Furthermore, the myeloid-origin group showed marked DNA fragmentation but less chromatin condensation during apoptosis. These results suggested that histone H1 determined chromatin structure and that its quantity affected the efficiencies of DNA fragmentation and chromatin condensation in apoptosis.

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D TuanI M London
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·X LiuX Wang
Mar 26, 2003·Cell Death and Differentiation·S NagataH Fukuyama
Apr 24, 2004·Methods in Molecular Biology·Demetrius MatassovZahra Zakeri
Sep 28, 2005·Biochemistry·Kohji HizumeKunio Takeyasu
May 23, 2006·Nature Structural & Molecular Biology·Frédéric CatezMichael Bustin
Feb 18, 2009·Blood·Daniel NowakH Phillip Koeffler
Aug 16, 2013·Nucleic Acids Research·Sean W HarshmanMichael A Freitas
Oct 17, 2013·PloS One·Hideaki TakataKazuhiro Maeshima
Jan 20, 2016·Genome Biology·Annalisa Izzo, Robert Schneider
Sep 23, 2016·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryo KoyamaRyushin Mizuta

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Citations

Nov 7, 2019·Clinical Epigenetics·I M KrzyzewskaM M A M Mannens
Jul 1, 2020·International Journal of Biological Macromolecules·Ilekuttige Priyan Shanura FernandoGinnae Ahn
Mar 30, 2021·Signal Transduction and Targeted Therapy·Pian YuXiang Chen

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis