Histone H2AX phosphorylation independent of ATM after X-irradiation in mouse liver and kidney in situ

Journal of Radiation Research
Manabu KoikeAki Koike

Abstract

Histone H2AX undergoes phosphorylation at Ser-139 (gamma-H2AX) rapidly in response to DNA double-strand breaks (DSBs) induced by ionizing radiation. The post-translational modification of H2AX plays a central role in responses to radiation, including the repair of DSBs. Although ataxia telangiectasia mutated (ATM) kinase phosphorylates Ser-139 of H2AX in vitro, the post-translational modification pattern and the modifier of H2AX in organs in vivo are not yet well understood. In this study, we detected phosphorylation of H2AX at Ser-139 in cells of the mouse ear, liver, and kidney after X-irradiation. Moreover, the phosphorylation of H2AX was regulated depending on not only the cell type, but also the organ type and the localization of a cell type in an organ. Following X-irradiation, H2AX was phosphorylated in the liver and kidney of ATM gene knockout mice, suggesting that ATM kinase is not essential for phosphorylation of H2AX in these organs after X-irradiation in vivo.

References

Mar 15, 2001·Current Opinion in Cell Biology·D Durocher, S P Jackson
Mar 21, 2001·Nature Reviews. Genetics·D C van GentR Kanaar
Sep 26, 2001·The Journal of Biological Chemistry·S BurmaD J Chen
Jul 5, 2003·Journal of Radiation Research·Kayo YoshidaTakashi Morita
Jan 27, 2004·The Journal of Biological Chemistry·Irene M WardJunjie Chen
Jul 29, 2004·DNA Repair·Oscar Fernandez-CapetilloAndré Nussenzweig
Jan 6, 2006·Journal of Radiation Research·Manabu KoikeYoko Kohno
Jul 22, 2006·Biochemical and Biophysical Research Communications·Boris GavrilovNikolai Tomilin
Oct 10, 2007·Biochemical and Biophysical Research Communications·Manabu KoikeAki Koike

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Citations

Mar 20, 2009·International Journal of Radiation Biology·Nirmal BhogalRobert G Bristow
Apr 22, 2015·Cellular and Molecular Life Sciences : CMLS·Sami BenzinaXavier Gidrol
Nov 14, 2015·Archives of Toxicology·Shiyao ZhuZheng Dong
Mar 6, 2010·Amino Acids·Mark N CruickshankDaniela Ulgiati
Nov 15, 2012·The Journal of Veterinary Medical Science·Manabu KoikeAki Koike
Jul 27, 2011·Biochemical and Biophysical Research Communications·Manabu KoikeAki Koike

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Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.

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