Histone H3 N-terminal acetylation sites especially K14 are important for rDNA silencing and aging

Scientific Reports
Heng-hao XuYong Xue

Abstract

Histone variants and histone modifications are essential components in the establishment and maintenance of the repressed status of heterochromatin. Among these histone variants and modifications, acetylation at histone H4K16 is uniquely important for the maintenance of silencing at telomere and mating type loci but not at the ribosomal DNA locus. Here we show that mutations at H3 N-terminal acetylation site K14 specifically disrupt rDNA silencing. However, the mutant ion at H3K14R doesn't affect the recruitment of Pol II repressor RENT (regulator of nucleolar silencing and telophase exit) complex at the rDNA region. Instead, the CAF-1(chromatin assembly factor I) subunit Cac2 level decreased in the H3K14R mutant. Further experiments revealed that the single mutation at H3K14 and multi-site mutations at H3 N-terminus including K14 also delayed replication-depend nucleosome assembly and advanced replicative life span. In conclusion, our data suggest that histone H3 N-terminal acetylation sites especially at K14 are important for rDNA silencing and aging.

References

Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L M JohnsonM Grunstein
Jan 15, 1997·Genes & Development·J S Smith, J D Boeke
Jan 15, 1997·Genes & Development·M BrykM J Curcio
Nov 14, 1997·The EMBO Journal·C E FritzeR Easton Esposito
Apr 5, 2003·Annual Review of Biochemistry·Laura N RuscheJasper Rine
Jul 30, 2004·Molecular and Cellular Biology·Jason C TannyDanesh Moazed
Jan 7, 2006·Nucleic Acids Research·Joel D NelsonKarol Bomsztyk
Feb 12, 2008·Current Protocols in Molecular Biology·Angie L BookoutMartha F Kramer
Feb 17, 2009·Genome Research·Hailiang HuangJoel S Bader
Jun 12, 2009·Nature·Weiwei DangShelley L Berger
Jun 27, 2009·Journal of Visualized Experiments : JoVE·Kristan K SteffenMatt Kaeberlein
Mar 2, 2010·Molecular Cell·Rebecca J BurgessZhiguo Zhang
Apr 3, 2010·Journal of Applied Physiology·Matthias HahnGunnar Schotta
Aug 12, 2010·Cell Cycle·Rebecca J Burgess, Zhiguo Zhang
Sep 14, 2010·Molecular Cell·Jason FeserJessica K Tyler
Oct 27, 2010·Proceedings of the National Academy of Sciences of the United States of America·Whitney R LuebbenJennifer K Nyborg
Jul 14, 2011·Nucleic Acids Research·Nilanjana ChatterjeeBlaine Bartholomew
Nov 6, 2012·Genes & Development·Tasuku KitadaMichael Grunstein

❮ Previous
Next ❯

Citations

Aug 16, 2016·Genetics·Marc R Gartenberg, Jeffrey S Smith
Jul 27, 2018·Histochemistry and Cell Biology·Christian Schöfer, Klara Weipoltshammer
Mar 28, 2019·Frontiers in Genetics·Diego Molina-SerranoAntonis Kirmizis
Jul 31, 2019·Cells·Emiliano Matos-Perdomo, Félix Machín
Jan 7, 2021·International Journal of Molecular Sciences·Dominik Saul, Robyn Laura Kosinsky

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
acetylations
ChIP
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.