Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast

Nucleic Acids Research
Shota SuzukiYota Murakami

Abstract

In budding yeast, Set2 catalyzes di- and trimethylation of H3K36 (H3K36me2 and H3K36me3) via an interaction between its Set2-Rpb1 interaction (SRI) domain and C-terminal repeats of RNA polymerase II (Pol2) phosphorylated at Ser2 and Ser5 (CTD-S2,5-P). H3K36me2 is sufficient for recruitment of the Rpd3S histone deacetylase complex to repress cryptic transcription from transcribed regions. In fission yeast, Set2 is also responsible for H3K36 methylation, which represses a subset of RNAs including heterochromatic and subtelomeric RNAs, at least in part via recruitment of Clr6 complex II, a homolog of Rpd3S. Here, we show that CTD-S2P-dependent interaction of fission yeast Set2 with Pol2 via the SRI domain is required for formation of H3K36me3, but not H3K36me2. H3K36me3 silenced heterochromatic and subtelomeric transcripts mainly through post-transcriptional and transcriptional mechanisms, respectively, whereas H3K36me2 was not enough for silencing. Clr6 complex II appeared not to be responsible for heterochromatic silencing by H3K36me3. Our results demonstrate that H3K36 methylation has multiple outputs in fission yeast; these findings provide insights into the distinct roles of H3K36 methylation in metazoans, which have differen...Continue Reading

References

Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Aug 11, 2001·Science·T Jenuwein, C D Allis
Dec 12, 2001·Nature Genetics·Antoine H F M PetersThomas Jenuwein
Aug 24, 2002·Science·Thomas A VolpeRobert A Martienssen
Oct 19, 2002·Science·Amy FrappierScott J Carpenter
Jan 1, 2004·Molecular and Cellular Biology·Juliet L ReidKevin Struhl
Aug 27, 2005·Cell·Dmitry K PokholokRichard A Young
Oct 18, 2005·Molecular and Cellular Biology·Bhargavi RaoJason D Lieb
Jul 11, 2006·Nature·Yuriko HarigayaMasayuki Yamamoto
Nov 3, 2006·Genes & Development·Hemali P Phatnani, Arno L Greenleaf
Feb 10, 2007·Cell·Tomoyasu SugiyamaShiv I S Grewal
Apr 24, 2007·Nature Structural & Molecular Biology·Estelle NicolasShiv I S Grewal
Jan 30, 2008·Cell Structure and Function·Hiroshi KimuraNaohito Nozaki
Jun 11, 2008·Molecular and Cellular Biology·Michael L YoudellJane Mellor
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jan 6, 2010·Molecular and Cellular Biology·Ling-Ju LinMichael C Schultz
Mar 9, 2010·Current Opinion in Genetics & Development·Shiv Is Grewal
Sep 6, 2011·Nature Structural & Molecular Biology·Francisca E Reyes-TurcuShiv I S Grewal
Jan 24, 2012·Nature Reviews. Molecular Cell Biology·Eric J Wagner, Phillip B Carpenter

❮ Previous
Next ❯

Citations

Oct 30, 2016·Transcription·Shota SuzukiShinya Takahata
Apr 8, 2017·Cellular and Molecular Life Sciences : CMLS·Stephen L McDaniel, Brian D Strahl
Jan 29, 2019·Genome Biology and Evolution·Elise Parey, Anton Crombach
Feb 23, 2019·Nucleic Acids Research·Rajaraman GopalakrishnanFred Winston
Oct 8, 2020·Epigenetics & Chromatin·Saikat Bhattacharya, Jerry L Workman
Apr 3, 2021·Epigenetics & Chromatin·Jessica ConnacherLyn-Marié Birkholtz
Aug 16, 2021·The Journal of Biological Chemistry·Saikat BhattacharyaJerry L Workman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.