Histone H4R3 methylation catalyzed by SKB1/PRMT5 is required for maintaining shoot apical meristem

PloS One
Minghui YueShilai Bao

Abstract

The shoot apical meristem (SAM) is the source of all of the above-ground tissues and organs in post-embryonic development in higher plants. Studies have proven that the expression of genes constituting the WUSCHEL (WUS)-CLAVATA (CLV) feedback loop is critical for the SAM maintenance. Several histone lysine acetylation and methylation markers have been proven to regulate the transcription level of WUS. However, little is known about how histone arginine methylation regulates the expression of WUS and other genes. Here, we report that H4R3 symmetric dimethylation (H4R3sme2) mediated by SKB1/PRMT5 represses the expression of CORYNE (CRN) to maintain normal SAM geometrics. SKB1 lesion results in small SAM size in Arabidopsis, as well as down-regulated expression of WUS and CLV3. Up-regulation of WUS expression enlarges SAM size in skb1 mutant plants. We find that SKB1 and H4R3sme2 associate with the chromatin of the CRN locus to down-regulate its transcription. Mutation of CRN rescues the expression of WUS and the small SAM size of skb1. Thus, SKB1 and SKB1-mediated H4R3sme2 are required for the maintenance of SAM in Arabidopsis seedlings.

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Citations

Apr 20, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Qiuling LiShilai Bao
Apr 7, 2016·Biology Direct·Georgi K Marinov, Michael Lynch
Feb 11, 2015·Cellular and Molecular Life Sciences : CMLS·Nicole StopaDavid Shechter
Oct 8, 2016·Chemical Biology & Drug Design·Sen JiSheng-Yong Yang

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Methods Mentioned

BETA
PCR
transgenic
immunoprecipitation
ChIP
pull down
ChIP-seq

Software Mentioned

UCSC Genome Browser
SOAP2

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