Histone-induced damage of a mammalian epithelium: the role of protein and membrane structure

The American Journal of Physiology
T J KleineS A Lewis

Abstract

In a previous report [T. J. Kleine, A. Gladfelter, P. N. Lewis, and S. A. Lewis, Am. J. Physiol. 268 (Cell Physiol. 37): C1114-C1125, 1995], we found that the cationic DNA-binding proteins histones H4, H1, and H5 caused a voltage-dependent increase in the transepithelial conductance in rabbit urinary bladder epithelium. In this study, results from lipid bilayer experiments suggest that histones H5-H1 and H4 form variably sized conductive units. Purified fragments of histones H4 and H5 were used to determine the role of histone tertiary structure in inducing conductance. Isolated COOH- and NH2-terminal tails of histone H4, which are random coils, were inactive, whereas the central alpha-helical domain induced a conductance increase. Although the activities of the central fragment and intact histone H4 were in many ways similar, the dose-response relationships suggest that the isolated central domain was much less potent than intact histone H4. This suggests than the NH2- and COOH-terminal tails are also important for histone H4 activity. For histone H5, the isolated globular central domain was inactive. Thus the random-coil NH2- and COOH-terminal tails are important for H5 activity as well. These results indicate that histone mo...Continue Reading

References

Oct 3, 1977·European Journal of Biochemistry·C Crane-RobinsonJ Tom-Kun
Aug 1, 1978·European Journal of Biochemistry·F J AvilesE M Bradbury
Oct 1, 1977·The Journal of General Physiology·S A LewisJ M Diamond
Jul 29, 1975·Biochemistry·P N LewisC Crane-Robinson
Aug 3, 1992·European Journal of Pharmacology·R A CrucianiE F Stanley
Aug 1, 1991·Current Opinion in Cell Biology·M C Peitsch, J Tschopp
Mar 1, 1989·The American Review of Respiratory Disease·S MotojimaG J Gleich
May 1, 1988·Immunology Today·J D Young, C C Liu
Feb 15, 1988·European Journal of Biochemistry·P N LewisS Chan
Apr 1, 1971·Analytical Biochemistry·J S WhiteheadL M Shannon
May 1, 1995·The American Journal of Physiology·T J KleineS A Lewis
Dec 1, 1993·The American Journal of Physiology·C J TzanS A Lewis
Feb 15, 1994·Biochemistry·J D MamanJ Allan
May 1, 1993·The Journal of Membrane Biology·C J TzanS A Lewis
Dec 1, 1958·The Journal of Experimental Medicine·J G HIRSCH
Dec 1, 1971·The Journal of Membrane Biology·J Yonath, M M Civan

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Citations

Dec 12, 2012·American Journal of Respiratory and Critical Care Medicine·Simon T AbramsCheng-Hock Toh
May 6, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jamison J GrailerPeter A Ward
Aug 14, 2019·Proceedings of the National Academy of Sciences of the United States of America·Paul VulliamyKarim Brohi
Nov 3, 2011·Journal of Thrombosis and Haemostasis : JTH·A BrillDenisa D Wagner
Jun 28, 2011·Blood·Tobias A FuchsDenisa D Wagner
Feb 15, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Miriam KalbitzPeter A Ward
Nov 27, 2015·Mediators of Inflammation·Zhiheng XuYimin Li
Jul 30, 2014·Journal of Thrombosis and Haemostasis : JTH·F SemeraroC T Esmon
Aug 8, 2018·Journal of Cellular and Molecular Medicine·Peter SzatmaryRobert Sutton
Jan 1, 2014·Translational Respiratory Medicine·Peter A Ward, Jamison J Grailer
Aug 9, 2013·American Journal of Respiratory and Critical Care Medicine·Carlos A BarreroSalim Merali
Aug 18, 2018·Mucosal Immunology·Gopinath KasettyArne Egesten
May 26, 2017·Cell Death & Disease·Eleanor SilkDaqing Ma
Jan 11, 2021·Thrombosis and Haemostasis·Sylvie BouvierJean-Christophe Gris
Aug 16, 2019·Thrombosis Research·Imre Varjú, Krasimir Kolev
Dec 18, 2020·Nature Communications·Connor H O' MearaChristopher R Parish
Mar 25, 2020·Shock·Birte WeberMiriam Kalbitz
Dec 2, 2004·Journal of Molecular Biology·Joseph RosenbluhAbraham Loyter

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