Histone-like protein: a novel method for measuring stress in fish

Diseases of Aquatic Organisms
D Robinette, E Noga

Abstract

We assessed the effect of chronic stress using a group of potent, broad-spectrum antimicrobial polypeptides, called histone-like proteins (HLPs), which appear to be an important component of non-specific immunity in channel catfish Ictalurus punctatus skin. An enzyme-linked immunosorbent assay (ELISA) was developed to measure the predominant HLP (HLP-1) in channel catfish skin. Catfish were then exposed to a chronic stress consisting of overcrowding and elevated ammonia. Healthy unstressed fish had consistently high HLP-1 levels, but fish that had been stressed for 1 wk had significantly depressed HLP-1 levels; HLP-1 levels declined further in fish stressed for 3 or 4 wk. The time-dependent decline in HLP-1 levels was not accompanied by any gross signs of disease. In contrast to HLP-1 levels, antibacterial activity in the skin was significantly greater in fish stressed for 1 wk compared with unstressed fish; in addition, antibacterial activity was the same in fish that were unstressed or stressed for 3 or 4 wk. This suggests that other antibiotics besides HLP-1 may be induced in the skin, especially during early stages of stress, that may compensate for depressed HLP-1 levels. Our results indicate that chronic stress has a sign...Continue Reading

Citations

Mar 7, 2014·Pharmaceuticals·Jorge A Masso-Silva, Gill Diamond
Aug 3, 2010·International Journal of Radiation Biology·Badri N PandeyKaushala P Mishra
Mar 24, 2007·Brain Injury : [BI]·Samuel J Marwit, Peggy N Kaye
Nov 17, 2009·Developmental and Comparative Immunology·Jone CorralesEdward J Noga
Nov 6, 2007·Fish & Shellfish Immunology·Shona K Whyte
Sep 24, 2005·The FEBS Journal·Gudmundur BergssonGudmundur Hrafn Gudmundsson
May 13, 2008·Journal of Fish Diseases·A ColorniE J Noga

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