Histone methylation changes are required for life cycle progression in the human parasite Schistosoma mansoni

PLoS Pathogens
David RoquisRonaldo de Carvalho Augusto

Abstract

Epigenetic mechanisms and chromatin structure play an important role in development. Their impact is therefore expected to be strong in parasites with complex life cycles and multiple, strikingly different, developmental stages, i.e. developmental plasticity. Some studies have already described how the chromatin structure, through histone modifications, varies from a developmental stage to another in a few unicellular parasites. While H3K4me3 profiles remain relatively constant, H3K27 trimethylation and bivalent methylation show strong variation. Inhibitors (A366 and GSK343) of H3K27 histone methyltransferase activity in S. mansoni efficiently blocked miracidium to sporocyst transition indicating that H3K27 trimethylation is required for life cycle progression. As S. mansoni is a multicellular parasite that significantly affects both the health and economy of endemic areas, a better understanding of fluke developmental processes within the definitive host will likely highlight novel disease control strategies. Towards this goal, we also studied H4K20me1 in female cercariae and adults. In particular, we found that bivalent trimethylation of H3K4 and H3K27 at the transcription start site of genes is a landmark of the cercarial st...Continue Reading

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Citations

Feb 23, 2020·PLoS Neglected Tropical Diseases·Ester Alves MotaRenata Guerra-Sá
Feb 3, 2021·Wellcome Open Research·Ronaldo de Carvalho AugustoChristoph Grunau
Dec 11, 2020·Scientific Reports·Murilo S AmaralSergio Verjovski-Almeida
Mar 5, 2021·Journal of Proteomics·Natália Silva da TrindadeFernanda Janku Cabral
Mar 6, 2021·Helminthologia·R A Gomes AssençoR Guerra-Sá
Jul 17, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Aline Marrara PradoFernanda Janku Cabral

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Datasets Mentioned

BETA
PRJNA219440
PRJNA236156
SRR6307188
SRR6307189
SRR6307192
SRR6307193
SRX1592114
SRX1592113
SRX1592108
SRX1592115

Methods Mentioned

BETA
immunoprecipitation
ChIP-Seq
PCR
Assay
PCA
ChIP
histone

Software Mentioned

chromstaR
HCS
Bowtie2
samtools
FastQC
grep
Bowtie
R
PEAKRANGER
Salmon

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