PMID: 44390Nov 1, 1979

Histone-nonhistone protein complex reconstruction

Ukrainskiĭ biokhimicheskiĭ zhurnal
G Kh Bozhko, V A Shelestin

Abstract

A complex of chromatin proteins composed of five histon fractions and nine nonhiston fractions and stable in 2M NaCl solution was destructed by treating with urea, changing pH and decreasing the NaCl concentration, No reconstruction of the complex was observed after the said factors has been removed and the initial solvent composition restored. Nevertheless the reconstruction was detected after dissociation of the complex by desalting in the presence of DNA. The reconstruction was successful when stimulated both dimers and tetramers, the chromatin protein complex under study cannot be reconstructed by "self-organization", the only way being through the stage of nucleoproteid formation, and is similar in this respect to some other complex protein structures of the cell.

Related Concepts

Chromosomal Proteins, Non-Histone
Carmol
Plasma Protein Binding Capacity
Bos indicus
Histone H7
Chromatin
Hydrogen-Ion Concentration
Osmolality
Thymus Gland

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