Histone octamer function in vivo: mutations in the dimer-tetramer interfaces disrupt both gene activation and repression

The EMBO Journal
M S SantistebanM M Smith

Abstract

Within the core histone octamer each histone H4 interacts with each H2A-H2B dimer subunit through two binding surfaces. Tyrosines play a central role in these interactions with H4 tyrosines 72 and 88 contacting one H2A-H2B dimer subunit, and tyrosine 98 contacting the other. To investigate the roles of these interactions in vivo, we made site-directed amino acid substitutions at each of these tyrosine residues. Elimination of either set of interactions is lethal, suggesting that binding of the tetramer to both dimers is essential. Temperature-sensitive mutants were obtained through single amino acid substitutions at each of the tyrosines. The mutants show both strong positive and negative effects on transcription. Positive effects include Spt- and Sin-phenotypes resulting from mutations at each of the three tyrosines. One allele has a strong negative effect on the expression of genes essential for the G1 cell cycle transition. At restrictive temperature, mutant cells fail to express the CLN1, CLN2, SWI4 and SWI6 genes, and have reduced levels of CLN3 mRNA. These results demonstrate the critical role of histone dimer-tetramer interactions in vivo, and define their essential role in the expression of genes regulating G1 cell cycl...Continue Reading

References

Nov 1, 1978·Cell·A WorcelM L Wong
Jan 1, 1977·Journal of Bacteriology·C N Gordon, S C Elliott
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R G Nelson, W L Fangman
Dec 1, 1979·Cell·L HerefordD B Kaback
Nov 1, 1992·Trends in Genetics : TIG·F Winston, M Carlson
Jan 1, 1991·Methods in Cell Biology·M M Smith
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G ArentsE N Moudrianakis
Oct 11, 1990·Nucleic Acids Research·G AlmouzniA P Wolffe
Dec 11, 1990·Nucleic Acids Research·L LocklearJ R Davie
Dec 22, 1989·Cell·H E RichardsonS I Reed
Nov 3, 1989·Science·L H Hartwell, T A Weinert
Feb 1, 1988·Genes & Development·C D Clark-AdamsF Winston
Mar 1, 1988·The Journal of Cell Biology·M M Smith, V B Stirling
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G SimchenG R Fink
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D ShortleD Botstein
Mar 27, 1984·Biochemistry·P A SmithR Chalkley
May 1, 1981·Cell·L M HerefordC S McLaughlin
May 1, 1981·Genetics·M CarlsonD Botstein
May 1, 1982·Biochimie·C Michalski-ScriveM H Loucheux-Lefebvre
Aug 30, 1982·Biochimica Et Biophysica Acta·A P Butler, D E Olins

❮ Previous
Next ❯

Citations

Aug 14, 2001·Journal of Cellular Biochemistry·O TrianaG C Toro
Mar 17, 2004·Biochimica Et Biophysica Acta·Rimma BelotserkovskayaDanny Reinberg
May 10, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Kevin ShoularsBarry M Markaverich
Jul 28, 1999·FEMS Microbiology Reviews·J Pérez-Martín
Jun 26, 1998·Current Opinion in Cell Biology·P D Gregory, W Hörz
Jul 7, 2009·Nature Cell Biology·Rakesh Kumar SinghAkash Gunjan
Mar 5, 2010·Bioinformatics·Amin Moghaddas Gholami, Kurt Fellenberg
Jan 16, 1999·Nucleic Acids Research·A P Wolffe, J J Hayes
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Christopher M WoodJohn P Baldwin
Jul 13, 2000·Molecular and Cellular Biology·L GlowczewskiM M Smith
Jan 1, 2004·Molecular and Cellular Biology·Andrea A Duina, Fred Winston
Feb 17, 2005·Molecular and Cellular Biology·Eugenia Y XuJames R Broach
Dec 31, 2011·PLoS Computational Biology·Mithun BiswasJörg Langowski
Feb 22, 2012·Genetics·Oliver J Rando, Fred Winston
May 23, 2006·Nucleic Acids Research·Erica L Mersfelder, Mark R Parthun
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher D CarvinMichael P Kladde
Jun 16, 2004·Current Opinion in Genetics & Development·Rimma Belotserkovskaya, Danny Reinberg
Oct 6, 1998·Annual Review of Biochemistry·J L Workman, R E Kingston
Jan 11, 2007·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuko MatsubaraMasami Horikoshi
Oct 12, 2010·Epigenetics : Official Journal of the DNA Methylation Society·Rakesh Kumar Singh, Akash Gunjan
Aug 6, 2013·Hearing Research·Wanda S LaymanJian Zuo
Dec 5, 2006·Biophysical Journal·Shantanu SharmaNikolay V Dokholyan
Apr 5, 2002·Molecular Cell·Karen Adelman, John T Lis
Jun 24, 2004·Journal of Cellular Biochemistry·Michael A FreitasMark R Parthun
Oct 12, 2000·Nature·G Orphanides, D Reinberg
May 10, 2005·The Journal of Biological Chemistry·Stephen D FarrisAnton Krumm
Jun 13, 2006·The Journal of Biological Chemistry·Danny Reinberg, Robert J Sims
Nov 21, 2018·Life Science Alliance·Tao WangKarolin Luger
Jun 21, 2019·Nucleic Acids Research·Mengping LongToyotaka Ishibashi
Nov 28, 2020·Genome Génome / Conseil National De Recherches Canada·Dongbo DingToyotaka Ishibashi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Batten Disease

Batten Disease is a group of nervous system disorders known as neuronal ceroid lipofuscinosis. This feed focuses on neurobiological and neuropathological aspects of this disease.