Histone Recycling by FACT and Spt6 during Transcription Prevents the Scrambling of Histone Modifications

Cell Reports
Célia JeronimoFrançois Robert

Abstract

Genomic DNA is framed by additional layers of information, referred to as the epigenome. Epigenomic marks such as DNA methylation, histone modifications, and histone variants are concentrated on specific genomic sites, where they can both instruct and reflect gene expression. How this information is maintained, notably in the face of transcription, is not completely understood. Specifically, the extent to which modified histones themselves are retained through RNA polymerase II passage is unclear. Here, we show that several histone modifications are mislocalized when the transcription-coupled histone chaperones FACT or Spt6 are disrupted in Saccharomyces cerevisiae. In the absence of functional FACT or Spt6, transcription generates nucleosome loss, which is partially compensated for by the increased activity of non-transcription-coupled histone chaperones. The random incorporation of transcription-evicted modified histones scrambles epigenomic information. Our work highlights the importance of local recycling of modified histones by FACT and Spt6 during transcription in the maintenance of the epigenomic landscape.

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Citations

Apr 3, 2020·Genes & Development·Allison C Schier, Dylan J Taatjes
Jun 12, 2020·Nature Communications·Georg O M BobkovPatrick Heun
Sep 9, 2020·Nature Structural & Molecular Biology·Júlia TornéGeneviève Almouzni
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Oct 27, 2020·Nucleic Acids Research·Tim Formosa, Fred Winston
Oct 7, 2020·Molecular Cancer Research : MCR·Jeffrey C MartinJoyce E Ohm
May 1, 2021·International Journal of Molecular Sciences·Elodie LayatAline V Probst
Apr 24, 2021·Genes & Development·Olga ViktorovskayaFred Winston
May 8, 2021·Frontiers in Cell and Developmental Biology·Reuben FranklinSihem Cheloufi
Mar 24, 2021·Molecular and Cellular Biology·Ann K Hogan, Daniel R Foltz
Jun 20, 2021·Molecular Cell·Kristina ŽumerPatrick Cramer
Nov 18, 2021·The EMBO Journal·Ann K HoganDaniel R Foltz

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