PMID: 9531506Jun 11, 1998Paper

Histones and basic polypeptides activate Ca2+/cation influx in various cell types

The Biochemical Journal
Alessandra GamberucciA Benedetti

Abstract

Histone H2A (1-10 microg/ml) added to Ehrlich ascite cell suspensions promoted: (i) Ca2+ influx, but no apparent intracellular Ca2+ mobilization; (ii) plasma-membrane depolarization and Na+ influx in Ca2+-free medium, which were recovered by Ca2+ readmission; (iii) influx of other cations such as Ba2+, Mn2+, choline+ and N-methyl-d-glucamine+, but not of propidium+, ethidium bromide and Trypan Blue. H2A-induced Ca2+ influx and cell depolarization were: (i) blocked by La3+ and Gd3+, but not by various inhibitors of receptor-activated Ca2+-influx pathways/channels; (ii) mimicked by various basic polypeptides, with Mr>4000; (iii) prevented or reversed by polyanions such as polyglutamate or heparin; (iv) present in other cell types, such as Jurkat, PC12 and Friend erythroleukaemia cells, but virtually absent from rat hepatocytes and thymocytes. We conclude that cationic proteins/polypeptides, by interacting in a cell-specific manner with the cell surface, can activate in those cells putative non-selective Ca2+ channels and membrane depolarization.

Citations

Nov 3, 2011·Journal of Thrombosis and Haemostasis : JTH·A BrillDenisa D Wagner
Oct 30, 2013·European Journal of Immunology·David S Pisetsky
Nov 27, 2015·Mediators of Inflammation·Zhiheng XuYimin Li
Apr 5, 2017·The FEBS Journal·Christopher R LupferThirumala-Devi Kanneganti
Aug 8, 2018·Journal of Cellular and Molecular Medicine·Peter SzatmaryRobert Sutton
Jun 28, 2011·Blood·Tobias A FuchsDenisa D Wagner
Aug 9, 2013·American Journal of Respiratory and Critical Care Medicine·Carlos A BarreroSalim Merali
Jun 6, 2003·European Journal of Biochemistry·Rui WangLingyun Wu
Aug 16, 2019·Thrombosis Research·Imre Varjú, Krasimir Kolev
Mar 17, 2005·Life Sciences·Vani Ganapathy, Chennam Srinivasulu Shyamala Devi

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