Sep 1, 1976

Histones bind more tightly to bromodeoxyuridine-substituted DNA than to normal DNA

Nucleic Acids Research
S Y LinA D Riggs


Using a membrane filter assay, we have obtained results from both kinetic and competition experiments indicating that histones bind more strongly to bromodeoxyuridine-substituted DNA than to normal DNA. At 37 degrees C in our standard buffer of 0.2 M ionic strength, the rate of dissociation of histones H1, H2, and h4 from BrdU-substituted DNA is respectively 7, 4, and 2 times slower than it is from normal DNA. Competition experiments show an even greater difference between BrdU-substituted and normal DNA with respect to histone binding. The tighter binding of histones to BrdU-substituted DNA is of interest because of the known effects of BrdU on eukaryotic chromosome condensation and staining, virus induction, and the inhibition of differentiation.

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Mentioned in this Paper

DNA, Viral
Plasma Protein Binding Capacity
Cell Differentiation Process
Histone Binding
Metabolic Inhibition
Chromosome Condensation
Virus Activation
Histone H7

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