Histopathological and neurological features of Atg4b knockout mice

Veterinary Pathology
R ReadP Vogel

Abstract

This investigation found that genetic inactivation of mouse Atg4b, 1 of the 4 mammalian homologs of the autophagy-related gene Atg4, resulted in amorphous globular bodies in the neuropil of the deep cerebellar nuclei and adjacent vestibular nuclei but nowhere else in the brain or other tissues. The spheroid-like bodies in the deep cerebellar and vestibular nuclei showed heterogeneous composition, reactivity with anti-ubiquitin antibody, and staining characteristics of proteinaceous material. Atg4b-deficient (Atg4b (-/-)) mice also showed a mild but measurable impairment of motor performance on the Rotarod. Atg4b (-/-) mice produced by breeding heterozygous parents were produced at a slightly lower than expected ratio to heterozygous and wild-type siblings but showed no other clear abnormalities in a battery of screening tests. These findings appear to be different than those reported for inactivation of other Atg4 homologs, suggesting that these homologs have tissue-specific functions beyond redundancy.

References

Nov 26, 2002·The Journal of Biological Chemistry·Guillermo MariñoCarlos López-Otín
Oct 11, 2003·Developmental Cell·Daniel J KlionskyYoshinori Ohsumi
Nov 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Brian P ZambrowiczArthur T Sands
Nov 26, 2003·The Journal of Clinical Investigation·Xueping QuBeth Levine
Dec 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhenyu YueNathaniel Heintz
Jun 16, 2004·Cellular and Molecular Life Sciences : CMLS·G Mariño, C López-Otín
Nov 5, 2004·Nature·Akiko KumaNoboru Mizushima
May 4, 2005·The Journal of Cell Biology·Masaaki KomatsuTomoki Chiba
Sep 27, 2005·The Journal of Biological Chemistry·Kenji SugawaraFuyuhiko Inagaki
Dec 6, 2005·Journal of Molecular Biology·Taichi KumanomidouTakashi Yamane
Apr 18, 2007·Autophagy·Ruth Scherz-ShouvalZvulun Elazar
Apr 20, 2007·The Journal of Biological Chemistry·Guillermo MariñoCarlos López-Otín
Jul 17, 2007·The American Journal of Pathology·Guanghong LiaoXiaoning Bi
Aug 19, 2007·Journal of Autoimmunity·Ana LleoM Eric Gershwin
Nov 17, 2007·Cell Host & Microbe·Anthony OrvedahlBeth Levine
Mar 25, 2008·Hormones : International Journal of Endocrinology and Metabolism·Yogendra S Rajawat, Ioannis Bossis
Sep 17, 2008·Biochemical Society Transactions·Lorenzo GalluzziGuido Kroemer
Oct 18, 2008·Physiology·Wei-Lien Yen, Daniel J Klionsky
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Marlène DreuxFrancis V Chisari

❮ Previous
Next ❯

Citations

Nov 21, 2012·The Journal of Biological Chemistry·Roderick A M WilliamsGraham H Coombs
Oct 27, 2011·Disease Models & Mechanisms·Paul N SchofieldJohn P Sundberg
Nov 5, 2013·Biochemical and Biophysical Research Communications·Elisa TranUrban Emmenegger
Sep 3, 2013·Progress in Retinal and Eye Research·Yusuke MurakamiDemetrios G Vavvas
Jul 6, 2014·Autophagy·Pei-Feng LiuChih-Wen Shu
Mar 2, 2016·Veterinary Pathology·P VogelR Brommage
Aug 18, 2017·Cell Death Discovery·Abdeljabar El AndaloussiNahed Ismail
Sep 14, 2017·The Journal of Antibiotics·Tatsuro Maruyama, Nobuo N Noda
Jun 12, 2020·EMBO Molecular Medicine·David AlsinaNils-Göran Larsson
Oct 21, 2020·European Journal of Medicinal Chemistry·Gaoxia YangJie Liu

❮ Previous
Next ❯

Datasets Mentioned

BETA
OST264114

Methods Mentioned

BETA
gene-trapping
gene-trap
gene trap
phosphotransferase
fluorescence activated cell sorting
gene knockout
confocal microscopy
genotyping
PCR
lipidation

Software Mentioned

Statistica
Smartrod

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Related Papers

The Journal of Clinical Investigation
Guillermo MariñoCarlos López-Otín
The Journal of Clinical Investigation
Andreas Till, Suresh Subramani
Proceedings of the American Thoracic Society
Stefan W RyterAugustine M K Choi
© 2021 Meta ULC. All rights reserved