Histopathological and Ultrastructural Changes after Electroporation in Pig Liver Using Parallel-Plate Electrodes and High-Performance Generator

Scientific Reports
B López-AlonsoO Lucía

Abstract

Irreversible electroporation (IRE) has gained attention as a new non-thermal therapy for ablation with important benefits in terms of homogeneous treatment and fast recovery. In this study, a new concept of high voltage generator is used, enabling irreversible electroporation treatment in large tissue volume using parallel plates. Unlike currently available generators, the proposed versatile structure enables delivering high-voltage high-current pulses. To obtain homogeneous results, 3-cm parallel-plates electrodes have also been designed and implemented. IRE ablation was performed on six female pigs at 2000 V/cm electric field, and the results were analysed after sacrifice three hours, three days and seven days after ablation. Histopathological and ultrastructural studies, including transmission and scanning electron microscopy, were carried out. The developed high-voltage generator has proved to be effective for homogeneous IRE treatment using parallel plates. The destruction of the membrane of the hepatocytes and the alterations of the membranes of the cellular organelles seem incompatible with cell death by apoptosis. Although endothelial cells also die with electroporation, the maintenance of vascular scaffold allows repai...Continue Reading

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Citations

Dec 19, 2019·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Matteo FiginiZhuoli Zhang
Jul 30, 2020·Radiology and Oncology·Tamara PolajzerDamijan Miklavcic
Feb 7, 2020·Annals of Biomedical Engineering·Borja MercadalAntoni Ivorra
Dec 23, 2020·The Journal of Clinical Investigation·Mohamed SalehGeorge K Gittes
Jun 3, 2021·Cancers·Alondra A AguilarChirag B Patel
Jun 20, 2021·Bioelectrochemistry·Tina Batista NapotnikDamijan Miklavčič

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Methods Mentioned

BETA
biopsies
electron microscopy

Software Mentioned

BodyParts3D
Aperio ImageScope - Pathology Slide Viewing
Leica
ImageScope

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis