Histopathological Correlations between Mediastinal Fat-Associated Lymphoid Clusters and the Development of Lung Inflammation and Fibrosis following Bleomycin Administration in Mice

Frontiers in Immunology
Yaser Hosny Ali ElewaYasuhiro Kon

Abstract

Bleomycin (BLM) has been reported to induce lung inflammation and fibrosis in human and mice and showed genetic susceptibility. Interestingly, the C57BL/6 (B6) mice had prominent mediastinal fat-associated lymphoid cluster (MFALCs) under healthy condition, and showed susceptibility to development of lung fibrosis following BLM administration. However, the pathogenesis of lung lesion progression, and their correlation with MFALC morphologies, remain to be clarified. To investigate the correlations between MFALC structures and lung injuries in B6 mice, histopathological examination of mediastinal fat tissues and lungs was examined at 7 and 21 days (d) following a single 50 μL intranasal (i.n.) instillation of either BLM sulfate (5 mg/kg) (BLM group) or phosphate-buffered saline (control group). The lung fibrosis was examined by Masson's trichrome (MT) stain of paraffin sections and mRNA expression levels of Col1a1, Col3a1, and Acta2 in different frozen lung samples. Furthermore, immunohistochemistry for CD3, B220, Iba1, Gr1, BrdU, LYVE-1, and peripheral node addressin (PNAd) was performed to detect T- and B-cells, macrophages, granulocytes, proliferating cells, lymph vessels (LVs), and high endothelial venules (HEVs). We found th...Continue Reading

References

Jan 1, 1992·Experimental Lung Research·S H Phan, S L Kunkel
Dec 1, 1986·American Journal of Clinical Pathology·T KanohM Yamagishi
Feb 1, 1986·British Journal of Haematology·M Peichev
Nov 1, 1971·Thorax·R W FleischmanR D Davis
Jan 1, 1969·Scandinavian Journal of Haematology·P StavemC B van der Hagen
Oct 1, 1984·British Journal of Haematology·M M Langenhuijsen
Sep 1, 1995·Immunology Today·J P Girard, T A Springer
Mar 24, 1999·Journal of Leukocyte Biology·H BiermannC Sunderkötter
Aug 15, 2001·Chest·S Sleijfer
Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noah S ButlerGary W Hunninghake
Oct 18, 2002·International Journal of Experimental Pathology·G IzbickiR Breuer
Dec 19, 2002·Annals of Oncology : Official Journal of the European Society for Medical Oncology·J M O'SullivanA Horwich
Sep 28, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·William G MartinStephen M Ansell
Feb 14, 2006·Seminars in Oncology·Mark MeadorsMichael C Perry
Jun 2, 2011·BMC Pulmonary Medicine·Effrosyni D ManaliNikolaos A Maniatis
May 16, 2013·American Journal of Respiratory Cell and Molecular Biology·Alexander V MisharinHarris Perlman
Oct 9, 2013·Respiratory Research·Wei LiuZi-Qiang Luo
May 24, 2014·Cell and Tissue Research·Yaser Hosny Ali ElewaYasuhiro Kon
May 16, 2015·The European Respiratory Journal·John HutchinsonTricia McKeever
Jul 7, 2015·Nature Immunology·Cécile BénézechJorge H Caamaño
Oct 2, 2015·The European Respiratory Journal·Ganesh RaghuSusanne Stowasser
Sep 2, 2016·Nature Communications·Lucy H Jackson-JonesCécile Bénézech
Feb 24, 2017·Nature Communications·Marissa J SchaferNathan K LeBrasseur
Apr 15, 2017·Frontiers in Physiology·Meng-Han LiuYu Ru Kou
Jul 12, 2017·The Journal of Experimental Medicine·Alexander V MisharinHarris Perlman
Sep 25, 2017·Journal of Inflammation·Tetsuya YokoyamaYoichi Nakanishi

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Citations

Apr 19, 2019·Experimental Biology and Medicine·Xuefeng XuJinglan Zhang
Dec 22, 2020·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Yaser H A ElewaYasuhiro Kon
May 7, 2021·Angiogenesis·Lucas Blanchard, Jean-Philippe Girard

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Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

ImageJ
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