History and results of the two inter-laboratory round robin endotoxin assay studies on cotton dust

American Journal of Industrial Medicine
David T W ChunHelle Wurtz

Abstract

In the US cotton industry, airborne cotton dust levels are regulated, and other countries are moving to specify safety limits for airborne endotoxins. There is concern about potential respiratory health hazards associated with agricultural and other organic dusts. In laboratories, ranking which samples have high and low levels of endotoxin is usually in good agreement between laboratories. When different laboratories assay identical samples, the levels differ. The objective of this research was to evaluate the intra- and inter-laboratory variability for 13 laboratories measuring endotoxin in cotton dust. Two inter-laboratory round robin endotoxin assay studies were conducted using cotton dust. In the first round robin, each laboratory used their normal in-house assay method and then used a common extraction protocol. In the second round robin, a common extraction protocol and endotoxin assay kit was used. The intra-laboratory results had small variations but inter-laboratory results had very high variations. The inter-laboratory results using a common extraction protocol showed reduced differences. Using the same extraction protocol and endotoxin assay kit, the intra-laboratory variation was small and inter-laboratory variation...Continue Reading

References

Sep 18, 2004·American Journal of Industrial Medicine·Robert R Jacobs, David Chun

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Mar 7, 2008·Archives of Toxicology·V LiebersT Brüning
Jul 13, 2013·Journal of Agromedicine·Stephen J ReynoldsTorben Sigsgaard
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