Histotypic mouse parietal cortex cultures: excitation/inhibition ratio and ultrastructural analysis

Journal of Neuroscience Methods
Pedro Benítez-DíazE Palacios-Prü

Abstract

Primary cultures of mouse parietal cortex, prepared between postnatal day 3 (P3) and P9, were studied using transmission electron microscopy and HPLC of excitatory (aspartate and glutamate) and inhibitory neurotransmitters (glycine, GABA and taurine) to determine their morphological and functional development. Relations between excitation and inhibition (E/I) were contrasted with ultrastructural features over the time course of in vitro development. After 6 days in vitro, cultured parietal cortex neurons prepared from mice at P3 had immature morphological characteristics, whereas P5 cultures showed a more developed histological structure but still with scarce synapses. The acquirement of histotypic characteristics was seen in P7 cultures, which contained numerous symmetric and asymmetric synaptic contacts. On P9, the cultures showed signs of tissue damage. In terms of neurotransmitter levels and E/I ratios, P7 cultures had relatively low E/I ratio as compared with the rest of the cultures prepared before or after P7. These results demonstrated that the development of inhibitory synaptic transmission, as indicated in the fall of E/I ratio, marked the maturation of cerebral cortical tissue and that the critical period to obtain h...Continue Reading

References

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May 1, 1982·Brain Research·E L Palacios-PrüL Palacios
Feb 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G J RamakersM A Corner
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Aug 18, 1999·Developmental Neuroscience·L Miranda-ContrerasE L Palacios-Prü
Mar 10, 2000·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·L Miranda-ContrerasE L Palacios-Prü
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Jul 5, 2001·Cellular and Molecular Life Sciences : CMLS·P Legendre
Sep 8, 2001·Cell and Tissue Research·L NguyenG Moonen
Jul 30, 2002·Developmental Neuroscience·Leticia Miranda-ContrerasErnesto Palacios-Prü
Nov 21, 2002·Hippocampus·Irina NikonenkoDominique Muller
Nov 14, 2003·Developmental Neuroscience·Pedro Benítez-DiazErnesto Palacios-Prü

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Citations

Jun 13, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Jingjing XuJiyang Dong

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