HIV-1 capture and antigen presentation by dendritic cells: enhanced viral capture does not correlate with better T cell activation

The Journal of Immunology : Official Journal of the American Association of Immunologists
Maria T Rodriguez-PlataJavier Martinez-Picado

Abstract

During HIV-1 infection, dendritic cells (DC) facilitate dissemination of HIV-1 while trying to trigger adaptive antiviral immune responses. We examined whether increased HIV-1 capture in DC matured with LPS results in more efficient Ag presentation to HIV-1-specific CD4(+) and CD8(+) T cells. To block the DC-mediated trans-infection of HIV-1 and maximize Ag loading, we also evaluated a noninfectious integrase-deficient HIV-1 isolate, HIV(NL4-3ΔIN). We showed that higher viral capture of DC did not guarantee better Ag presentation or T cell activation. Greater HIV(NL4-3) uptake by fully LPS-matured DC resulted in higher viral transmission to target cells but poorer stimulation of HIV-1-specific CD4(+) and CD8(+) T cells. Conversely, maturation of DC with LPS during, but not before, viral loading enhanced both HLA-I and HLA-II HIV-1-derived Ag presentation. In contrast, DC maturation with the clinical-grade mixture consisting of IL-1β, TNF-α, IL-6, and PGE(2) during viral uptake only stimulated HIV-1-specific CD8(+) T cells. Hence, DC maturation state, activation stimulus, and time lag between DC maturation and Ag loading impact HIV-1 capture and virus Ag presentation. Our results demonstrate a dissociation between the capacity t...Continue Reading

References

Sep 1, 1987·Reviews of Infectious Diseases·A G JohnsonE Ribi
Apr 1, 1998·Nature·J Banchereau, R M Steinman
Oct 25, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S Manickasingham, C Reis e Sousa
Jan 5, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jamila IsmailiPatrice M Dubois
Oct 2, 2002·Nature Biotechnology·Marielle CavroisWarner C Greene
Oct 22, 2002·Journal of Virology·Brenda L FredericksenJ Victor Garcia
Mar 1, 2003·Science·E Sergio TrombettaIra Mellman
May 6, 2003·Science·David McDonaldThomas J Hope
Oct 25, 2003·Blood·Arnaud MorisOlivier Schwartz
Nov 25, 2003·Blood·Stuart G TurvilleAnthony L Cunningham
Apr 2, 2005·Nature Reviews. Immunology·Jacques Banchereau, A Karolina Palucka
Jan 28, 2006·Journal of Virology·Marielle CavroisWarner C Greene
Feb 21, 2006·Nature·J Magarian Blander, Ruslan Medzhitov
Oct 26, 2006·Nature Reviews. Immunology·Li Wu, Vineet N KewalRamani
Nov 23, 2006·Nature Medicine·Jason M BrenchleyDaniel C Douek
Dec 6, 2006·The Journal of Experimental Medicine·Marjorie PionVincent Piguet
May 4, 2007·Journal of Virology·Nuria Izquierdo-UserosJavier Martinez-Picado
Sep 14, 2007·Immunological Reviews·Hideki UenoJacques Banchereau
Dec 20, 2008·Journal of Internal Medicine·C R Rinaldo
May 1, 2007·Current Opinion in HIV and AIDS·Viswanathan LakshmananNina Bhardwaj
Jun 3, 2009·Trends in Molecular Medicine·Jeffrey D Ahlers, Igor M Belyakov
Jul 18, 2009·European Journal of Immunology·Jeffrey D Ahlers, Igor M Belyakov
Apr 3, 2010·PLoS Pathogens·Nuria Izquierdo-UserosJavier Martinez-Picado
Jul 20, 2010·Immunotherapy·Telma Miyuki OshiroAlberto José da Silva Duarte

❮ Previous
Next ❯

Citations

Nov 13, 2012·PloS One·Carmen Álvarez-FernándezSonsoles Sánchez-Palomino
Sep 9, 2017·International Journal of Cancer. Journal International Du Cancer·Florence FaureSebastian Amigorena
Apr 1, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina HertoghsTeunis B H Geijtenbeek
May 1, 2015·Journal of Virology·Diana AyindeOlivier Schwartz
Jul 15, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linda M van den BergTeunis B H Geijtenbeek
Jun 30, 2015·PLoS Pathogens·Wilfried PoschDoris Wilflingseder
Jun 12, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pierre-Grégoire CoulonArnaud Moris
Mar 3, 2020·EBioMedicine·Prasanta K DashHoward E Gendelman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Avian Influenza: Innate Immune Adjuvant

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Absence Epilepsy

Absence epilepsy is a common seizure disorder in children which can produce chronic psychosocial sequelae. Discover the latest research on absence epilepsies here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Avian Influenza: Innate Immune Adjuvant (ASM)

Adjuvants systems that are added to vaccines against avian influenza have be explored to enhance the innate immune system response against the virus. Here is the latest research on avian influenza and the innate immune adjuvant.

Ataxia telangiectasia (MDS)

Ataxia telangiectasia is a rare neurodegenerative diseases caused by defects in the ATM gene, which is involved in DNA damage recognition and repair pathways. Here is the latest research on this autosomal recessive disease.