HIV-1 DNA burden dynamics in CD4 T cells and monocytes in patients undergoing a transient therapy interruption

Journal of Medical Virology
Anna Rosa GarbugliaMaria Rosaria Capobianchi

Abstract

Replication-competent HIV, as well as HIV-1 DNA, has been detected in CD4 T cells and in monocytes during antiretroviral therapy (ART), indicating that these cells could represent an important viral reservoir. We measured HIV-1 DNA in monocytes and CD4 T cells in patients undergoing transient therapy interruption (TTI), to establish the dynamic of HIV-1 DNA burden and to find possible correlations with immune restoration and re-establishment of virological control after ART resumption. In most patients CD4 depletion and viral load rebound followed TTI. Rapid resumption of virological and immunological control was achieved after ART reintroduction. After TTI, in most cases a transient increase of both monocyte and CD4 HIV-1 DNA burden was observed. After ART reintroduction, both CD4 T cell and monocyte HIV-1 DNA copy number decreased, reaching baseline levels at the end of observation. At this time monocyte HIV-1 DNA burden was always undetectable, while CD4 T cell HIV-1 DNA burden was lower than at baseline. As CD4 T cell HIV-1 DNA values are independently associated with CD4 depletion, the increase of HIV-1 DNA burden in these cells after TTI is presumably due to acute infection, causing cell death. This is also supported by t...Continue Reading

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Citations

May 12, 2010·AIDS Research and Human Retroviruses·Milena NasiAndrea Cossarizza
Mar 29, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Ferdinando DianzaniMaria R Capobianchi
Sep 8, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M C ReD Gibellini
Jul 20, 2018·The Journal of Clinical Investigation·Radwa SharafJonathan Z Li
Jul 1, 2006·Expert Review of Clinical Immunology·Stephanie Venzke, Oliver T Keppler
Jul 13, 2019·Frontiers in Immunology·Michelle E WongAnna C Hearps
Mar 3, 2021·Seminars in Immunology·Rebecca T VeenhuisJanice E Clements

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