HIV-1-encoded antisense RNA suppresses viral replication for a prolonged period.

Retrovirology
Mie Kobayashi-IshiharaToshiki Watanabe

Abstract

Recent evidence proposes a novel concept that mammalian natural antisense RNAs play important roles in cellular homeostasis by regulating the expression of several genes. Identification and characterization of retroviral antisense RNA would provide new insights into mechanisms of replication and pathogenesis. HIV-1 encoded-antisense RNAs have been reported, although their structures and functions remain to be studied. We have tried to identify and characterize antisense RNAs of HIV-1 and their function in viral infection. Characterization of transcripts of HEK293T cells that were transiently transfected with an expression plasmid with HIV-1NL4-3 DNA in the antisense orientation showed that various antisense transcripts can be expressed. By screening and characterizing antisense RNAs in HIV-1NL4-3-infected cells, we defined the primary structure of a major form of HIV-1 antisense RNAs, which corresponds to a variant of previously reported ASP mRNA. This 2.6 kb RNA was transcribed from the U3 region of the 3' LTR and terminated at the env region in acutely or chronically infected cell lines and acutely infected human peripheral blood mononuclear cells. Reporter assays clearly demonstrated that the HIV-1 LTR harbours promoter acti...Continue Reading

References

Sep 15, 1989·Biochemical and Biophysical Research Communications·D LaroccaT K Brunck
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T M FolksA S Fauci
Apr 5, 2002·Advances in Genetics·E Gerhart H WagnerPascale Romby
Sep 1, 2004·Archives of Virology·K BentleyM Churchill
Sep 11, 2004·Current Opinion in Microbiology·Alexandra Trkola
Dec 14, 2004·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hans Peter Luther
Sep 6, 2005·Science·S KatayamaUNKNOWN FANTOM Consortium
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yorifumi SatouMasao Matsuoka
Mar 4, 2006·Retrovirology·Marie-Hélène CavanaghBenoit Barbeau
Aug 5, 2006·The Journal of Biological Chemistry·Yamina BennasserKuan-Teh Jeang
Oct 4, 2007·Retrovirology·Sébastien LandryBenoit Barbeau
Apr 4, 2008·Nucleic Acids Research·Gudrun Bjornsdottir, Lawrence C Myers
Sep 17, 2008·Molecular and Cellular Biology·Nathalie BerteauxEric Adriaenssens
Feb 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yasnory T F SasakiTetsuro Hirose
Feb 24, 2009·Microbes and Infection·Makoto YamagishiToshiki Watanabe
Jul 30, 2009·Nature Reviews. Molecular Cell Biology·Mohammad Ali Faghihi, Claes Wahlestedt
Aug 5, 2009·Retrovirology·Masao Matsuoka, Patrick L Green
Sep 2, 2009·Proceedings of the National Academy of Sciences of the United States of America·Emiko Matsuda, David J Garfinkel
Dec 25, 2009·Retrovirology·Evelyn M KilareskiBrian Wigdahl
Jan 13, 2010·The Journal of Experimental Medicine·Anju BansalPaul A Goepfert
Feb 5, 2010·Journal of Virology·Mads Heilskov RasmussenFinn Skou Pedersen
Feb 23, 2010·Microbes and Infection·Ariko MiyakeRyouichi Horie
Mar 6, 2010·The New Phytologist·J GeorgW R Hess

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Citations

Oct 9, 2012·The Journal of Biological Chemistry·Zachary KlaseKuan-Teh Jeang
Feb 22, 2013·Journal of Virology·Cynthia TorresillaBenoit Barbeau
Jun 28, 2012·BMC Biology·Kuan-Teh Jeang
Apr 6, 2013·Retrovirology·Jerome A ZackDimitrios N Vatakis
Dec 21, 2012·Retrovirology·Yong-Hui ZhengKenzo Tokunaga
Jul 3, 2013·Viruses·Jessica N Groen, Kevin V Morris
Jul 6, 2013·AIDS Research and Human Retroviruses·Jessica M MatesJesse J Kwiek
Mar 1, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sheena SaaymanKevin V Morris
Feb 22, 2013·Current Opinion in HIV and AIDS·Angela Ciuffi, Amalio Telenti
Dec 26, 2015·International Journal of Molecular Sciences·Shudai LinXiquan Zhang
Apr 14, 2015·Expert Opinion on Biological Therapy·Sheena SaaymanMarc S Weinberg
Jul 30, 2015·Virus Research·Daniel C LazarSheena M Saayman
Oct 11, 2015·Virus Research·Puri Fortes, Kevin V Morris
Apr 10, 2016·Nucleic Acids Research·Marc S Weinberg, Kevin V Morris
May 11, 2013·Current Opinion in Virology·Sebastian EilebrechtOlivier Rohr
Sep 7, 2014·Biochemical and Biophysical Research Communications·Hazuki Takahashi, Piero Carninci
Feb 24, 2015·Trends in Microbiology·Benoit Barbeau, Jean-Michel Mesnard
Dec 12, 2012·Virology·David M KnipeThomas M Kristie
Jan 28, 2015·Molecular Therapy. Nucleic Acids·Kazuo SuzukiAnthony D Kelleher
Jan 8, 2015·Current Opinion in HIV and AIDS·Alex HarwigBen Berkhout
Apr 21, 2018·Viruses·Craig MillerSue VandeWoude
Sep 13, 2017·Antimicrobial Agents and Chemotherapy·Na ZhaoBen Berkhout
Feb 11, 2018·Retrovirology·Xu ZhangYijun Zhang
Mar 3, 2020·Frontiers in Microbiology·Juliette SavoretAntoine Gross
Sep 25, 2018·Frontiers in Immunology·Veron RamsuranSmita Kulkarni
Jun 12, 2018·Frontiers in Microbiology·Mie Kobayashi-IshiharaYasuko Tsunetsugu-Yokota
Oct 6, 2018·Retrovirology·Catalina MéndezAnthony D Kelleher
Feb 13, 2020·Seminars in Immunopathology·Ulrike C LangeCarine Van Lint
Sep 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Elodie CassanOlivier Gascuel
Jan 16, 2017·Virology Journal·Mamneet MangheraRenée N Douville
Jul 31, 2018·AIDS Research and Human Retroviruses·Sergio Castro-GonzalezRuth Serra-Moreno
Oct 1, 2020·Viruses·Aneta PlutaRenée N Douville
Dec 9, 2020·The Journal of Clinical Investigation·Elizabeth J Hennessy, Garret A FitzGerald
Jan 30, 2021·Frontiers in Microbiology·Juliette SavoretNathalie Chazal
Nov 14, 2020·Viruses·Koh Fujinaga, Daniele C Cary
Jun 3, 2021·Vaccines·Zahra GholizadehFabio Romerio

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Datasets Mentioned

BETA
M19921.2
JQ866626

Methods Mentioned

BETA
PCR
electrophoresis
transfection
PCRs
Assay

Software Mentioned

Genetyx
ORF Finder
TFsearch
fold
HCpolya

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