HIV-1 Envgp140 trimers elicit neutralizing antibodies without efficient induction of conformational antibodies

Vaccine
Joseph F BowerTed M Ross

Abstract

Currently, no vaccine for human immunodeficiency virus (HIV-1) provides protection from virus infection. One reason for these disappointing results has been the difficulty of current vaccine candidates to elicit high-titer, broadly reactive immunity to a large number of viral proteins. Recently, our laboratory demonstrated that the coupling of C3d to a soluble trimerized HIV-1 envelope (Env(gp140(FT))) elicited higher titers of neutralizing antibodies than monomers of Env(gp120) coupled to C3d [Bower JF, Yang X, Sodroski J, Ross TM. Elicitation of neutralizing antibodies with DNA vaccines expressing soluble stabilized human immunodeficiency virus type 1 envelope glycoprotein trimers conjugated to C3d. J Virol 2004;78(9):4710-9]. To determine if the induction of conformational antibodies correlated with neutralization, mice (BALB/c) were primed (2x) with DNA plasmids expressing monomeric Env(gp120) or trimeric Env(gp140) alone or fused to mC3d(3) at one of two doses (2.0microg or 0.2microg), followed by a boost of recombinant uncleaved, trimeric Env(gp140). Regardless of the priming dose of DNA, all mice had high-titer anti-Env IgG antibodies. Interestingly, Env(gp140) trimers did not elicit higher titers of antibodies that reco...Continue Reading

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Citations

Mar 7, 2008·Proceedings of the National Academy of Sciences of the United States of America·Gary FreyBing Chen
Sep 1, 2007·Current Opinion in HIV and AIDS·Heather B Jaspan, Willem A Hanekom
Jun 1, 2010·Vaccine·Mark D HicarJames E Crowe
Nov 7, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yajing ChenYuxing Li
Jan 11, 2008·AIDS Research and Human Retroviruses·Lianxing LiuYiming Shao
Jul 26, 2018·Scientific Reports·M CascalhoJ L Platt

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