HIV-1 Gag-specific immunity induced by a lentivector-based vaccine directed to dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America
Bingbing DaiPin Wang

Abstract

Lentivectors (LVs) have attracted considerable interest for their potential as a vaccine delivery vehicle. In this study, we evaluate in mice a dendritic cell (DC)-directed LV system encoding the Gag protein of human immunodeficiency virus (HIV) (LV-Gag) as a potential vaccine for inducing an anti-HIV immune response. The DC-directed specificity is achieved through pseudotyping the vector with an engineered Sindbis virus glycoprotein capable of selectively binding to the DC-SIGN protein. A single immunization by this vector induces a durable HIV Gag-specific immune response. We investigated the antigen-specific immunity and T-cell memory generated by a prime/boost vaccine regimen delivered by either successive LV-Gag injections or a DNA prime/LV-Gag boost protocol. We found that both prime/boost regimens significantly enhance cellular and humoral immune responses. Importantly, a heterologous DNA prime/LV-Gag boost regimen results in superior Gag-specific T-cell responses as compared with a DNA prime/adenovector boost immunization. It induces not only a higher magnitude response, as measured by Gag-specific tetramer analysis and intracellular IFN-gamma staining, but also a better quality of response evidenced by a wider mix of c...Continue Reading

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Citations

May 17, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Bingbing DaiPin Wang
Apr 17, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jingying ZhouZhiwei Chen
Jan 21, 2011·Human Gene Therapy·Alexander AgeichikMary K Collins
May 3, 2013·The Journal of Clinical Investigation·Jingying ZhouZhiwei Chen
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Jan 5, 2011·Immunological Reviews·Biliang HuPin Wang
Mar 12, 2011·The Journal of Gene Medicine·Luciene LopesMary K Collins
Apr 18, 2012·The Biochemical Journal·Toshie SakumaYasuhiro Ikeda
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Aug 1, 2015·Molecular Therapy. Methods & Clinical Development·Peirong HuTal Kafri
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Nov 20, 2015·Journal of Immunology Research·Cleo Goyvaerts, Karine Breckpot
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Jul 3, 2021·Vaccines·Joseph HokelloMudit Tyagi
Oct 9, 2021·Expert Review of Vaccines·Min-Wen KuLaleh Majlessi

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