HIV-1 Genetic Diversity and High Prevalence of Pretreatment Drug Resistance in Tianjin, China.

AIDS Research and Human Retroviruses
Rui ZengPing Ma

Abstract

Diversity of genotypes and prevalence of pretreatment drug resistance (PDR) are challenges for the epidemic control and vaccine development of HIV-1. However, little is known about the situation in Tianjin. Blood samples were collected from newly diagnosed, antiretroviral treatment (ART)-naive HIV/AIDS patients from January 2016 to November 2019. The target fragment in the pol gene was sequenced after RNA extraction and gene amplification. The HIV-1 genotype was identified by phylogenetic analysis. Drug resistance was carried out using the Stanford University HIVdb algorithm. A total of 305 pol sequences from 279 non-PDR individuals and 35 PDR individuals were successfully amplified. The most prevalent genotype was CRF01_AE (65.6%, 200/305), followed by CRF07_BC (22.0%, 67/305) and B (3.0%, 9/305). A variety of circulating recombinant forms (CRFs) and unique recombinant forms were found. The overall incidence of PDR was 11.5% (35/305), with 9.8% (30/305) to non-nucleoside reverse transcriptase (RT) inhibitors (NNRTIs). The most frequent mutation pattern against NNRTIs was V179D/E/T (6.9%, 21/305), with M184V (1.0%, 3/305) and K65R (1.0%, 3/305) against nucleoside RT inhibitors (NRTIs). M64L (0.1%, 1/305) was the sole mutation f...Continue Reading

References

Apr 15, 2000·Science·D L RobertsonB Korber
Nov 1, 2011·The Lancet Infectious Diseases·Raph L HamersUNKNOWN PharmAccess African Studies to Evaluate Resistance (PASER) Investigators
Oct 30, 2012·The Journal of Infection·Joris Hemelaar
Jul 19, 2014·International Journal of STD & AIDS·Li ZhangJing Wang
Mar 27, 2018·Journal of Medical Virology·Zhi-Wei ChenZhiwei Chen
Nov 21, 2019·Southern African Journal of HIV Medicine·Mohendran ArcharyAmy Slogrove

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Citations

Dec 15, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yun LanFengyu Hu

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Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

REGA HIV - 1 Subtyping Tool
Clustal X
BioEdit
SPSS
MEGA X

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