HIV-1 gp120-induced neuroinflammation: relationship to neuron loss and protection by rSV40-delivered antioxidant enzymes

Experimental Neurology
Jean-Pierre LouboutinDavid S Strayer

Abstract

HIV-1 gp120 neurotoxicity and oxidant injury are well documented, but consequent neuroinflammation is less understood. Rat caudate-putamens (CPs) were challenged with 100-500 ng HIV-1BaL gp120, with or without prior rSV40-delivered superoxide dismutase or glutathione peroxidase. CD11b-positive microglia were increased 1 day post-challenge; Iba-1- and ED1-positive cells peaked at 7 days and 14 days. Astrocyte infiltration was maximal at 7-14 days. MIP-1alpha was produced immediately, mainly by neurons. ED1- and GFAP-positive cells correlated with neuron loss and gp120 dose. We also tested the effect of more chronic gp120 exposure on neuroinflammation using an experimental model of continuing gp120 exposure. SV(gp120), a recombinant SV40-derived gene transfer vector was inoculated into the rat CP, leading to chronic expression of gp120, ongoing apoptosis in microglia and neurons, and oxidative stress. Increase in microglia and astrocytes was seen following intra-CP SV(gp120) injection, suggesting that continuing gp120 production increased neuroinflammation. SV(SOD1) or SV(GPx1) significantly reduced MIP-1alpha and limited neuroinflammation following gp120 administration into the CP, as well as microglia and astrocytes proliferati...Continue Reading

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Citations

Aug 21, 2010·Immunologic Research·K Grovit-Ferbas, M E Harris-White
Mar 27, 2012·TheScientificWorldJournal·Jean-Pierre Louboutin, David S Strayer
Dec 18, 2013·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Isabella Cristina Gomes BarretoElisabete Castelon Konkiewitz
Nov 10, 2013·Immunologic Research·Rebeca Geffin, Micheline McCarthy
Oct 29, 2011·Journal of Cellular Physiology·Patrick M ReganKamel Khalili
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Nov 27, 2016·Glia·Natalie C ChenJulio Martín-García
Nov 19, 2011·The European Journal of Neuroscience·Jean-Pierre LouboutinDavid S Strayer
Jul 11, 2018·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Lester J Rosario-RodríguezLoyda M Meléndez
Aug 6, 2021·Journal of Neurovirology·Ferzana Hassan AmodAnand Moodley
Aug 26, 2021·Retrovirology·E A Nickoloff-BybelP J Gaskill

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