Abstract
We have developed an in vitro reconstructed vaginal mucosa integrating Langerhans cells (LCs), obtained by differentiation of umbilical cord blood CD34(+) hematopoietic progenitor cells, and, in this model, we have investigated the infection of LCs by human immunodeficiency virus type 1 (HIV-1). Proviral DNA of X4 (LAI and NL4-3) and R5 (Ba-L) HIV-1 strains were detected in LCs integrated in the reconstituted mucosa. Infection of LCs could be specifically inhibited by the chemokines stromal cell-derived factor 1 (SDF-1) and RANTES (regulated on activation, normally T cell-expressed and -secreted), confirming the presence of functional coreceptors on LCs generated in vitro. A complete inhibition of LCs, by use of azidothymidine, a reverse-transcriptase inhibitor, was also observed. Moreover, HIV-1-infected LCs of the reconstructed mucosa were able to transmit R5 or X4 HIV-1 strains to activated peripheral blood mononuclear cells. Such a model could be a useful tool to study the mechanisms involved in transmission of HIV in the female genital tract.
Citations
Mar 24, 2010·Immunology and Cell Biology·Michiel van der Vlist, Teunis B H Geijtenbeek
Jun 24, 2010·Mucosal Immunology·Y GanorM Bomsel
Sep 27, 2005·AIDS·Kevin K AriënGuido Vanham
Jun 27, 2008·AIDS·Marielle BouschbacherJenny Valladeau
Dec 15, 2006·Journal of Virology·Paul U CameronSharon R Lewin
Apr 20, 2007·Journal of Virology·Kelly M FahrbachThomas J Hope
Jul 5, 2005·Journal of Virology·Kevin K AriënEric J Arts
Aug 4, 2006·The Journal of Clinical Investigation·Lucile EspertMartine Biard-Piechaczyk
Nov 28, 2013·Scientifica·Charles R Rinaldo
Dec 13, 2006·The Journal of Investigative Dermatology·Yannick ChâteauLaurent Misery
May 15, 2007·Fertility and Sterility·Osmond J D'Cruz, Fatih M Uckun
Aug 3, 2013·Reviews in Medical Virology·Andrew N HarmanPaul U Cameron
Feb 18, 2015·Advanced Drug Delivery Reviews·Rita Monteiro MachadoRita Palmeira-de-Oliveira
Jul 18, 2006·Antiviral Research·Graciela Andrei
Jan 3, 2006·Virology·Kevin K AriënGuido Vanham
Jul 5, 2016·The Journal of Investigative Dermatology·Magdalena A CzubalaVincent Piguet
Jul 11, 2014·AIDS·Vincent PavotStéphane Paul
Jul 30, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Najla NasrAnthony L Cunningham
Sep 29, 2011·Alternatives to Laboratory Animals : ATLA·Gertrude-Emilia CostinRoger D Curren
Jul 6, 2014·Retrovirology·Ramin Sarrami-ForooshaniTeunis B H Geijtenbeek
Oct 9, 2021·Clinical Science·Laura MarongiuFrancesca Granucci